HPLC法测定首乌益智胶囊中大黄素和大黄素甲醚的含量

建立了高效液相色谱法(HPLC)同时测定首乌益智胶囊中大黄素和大黄素甲醚含量的方法。采用C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇-0.1%磷酸水(82:18,V/V)为流动相,检测波长为289 nm,流速为1.0 m L/min,柱温30℃。测得大黄素在0.019~0.19μg(R=0.999 9,n=5)、大黄素甲醚在0.021~0.21μg(R=1.000 0,n=5)线性关系良好;大黄素和大黄素甲醚的平均回收率分别为98.58%、100.01%,相对标准偏差分别为2.66%、1.15%。研究表明,该方法专属性强、结果准确,可作为同时测定首乌益智胶囊中大黄素和大黄素甲醚含量的方法。

HPLC based content determination of emodin and emodin monomenthyl ether from Shouwuyizhi capsule

We constructed a HPLC based content determination method for emodin and emodin monomenthyl ether from Shouwuyizhi capsule. Samples were separated by C18 (250 nm×4.6 nm, 5 μm) column with methanol 0.1% phosphoric acid water (82:18, V/V) as mobile phase. Determination conditions were determination wavelength of 289 nm, flow rate of 1.0 mL/min, and column temperature of 30 ℃. Better linear relationship exists in the range 0.019~0.19 μg（R=0.999 9,n=5) for emodin, and in the range 0.021~0.21 μg（R=1.000 0，n=5）for emodin monomenthyl ether. Their average recovery rates are respectively 98.58% and 100.01%, and RSDs are 2.66% and 1.15%. Results show that the method has strong specificity and accuracy, so it can be applied to simultaneous content determination of emodin and emodin monomenthyl ether from Shouwuyizhi capsule.