高速逆流色谱法连续分离制备小白菊内酯

高速逆流色谱法连续进样,分离制备杭白菊中的小白菊内酯?采用高速逆流色谱法进行分离,以正已烷-乙酸乙酯-甲醇-水(5∶5∶6∶4,V/V)为溶剂系统,上相为固定相,下相为流动相,连续进样3次,浸膏进样量330?350?370 mg,转速800 r/min,流速2.0 mL/min,检测波长254 nm?从杭白菊超声提取物中,分离得到小白菊内酯5.0?5.2?5.5mg,纯度分别为93.1%?92.6%?92.9%?利用连续进样的高速逆流色谱法,可高效分离和纯化杭白菊中的小白菊内酯?

Continuous separation and preparation of parthenolide from Chrysanthemum morifolium with high-speed counter-current chromatography

We separated and prepared parthenolide from Chrysanthemum morifolium with continuous multiple preparation and high-speed counter-current chromatography ( HSCCC) . We acquired parthenolide of 5.0, 5.2 and 5.5 mg from Chrysanthemum morifolium extract of 330, 350 and 370 mg. Their purities were respectively 93. 1%, 92. 6%, and 92. 9%. Experimental conditions were HSCCC, solvent system of n-hexane-ethyl acetate-methanol-water ( 5∶5∶6∶4, V/ V) , upper stationary phase and lower mobile phase, rotation speed of 800.0 r/ min, flow rate of 2.0 mL/ min and detection wavelength of 254 nm. It shows that continuous preparation HSCCC can efficiently separate and purify parthenolide from Chrysanthemum morifolium.