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生物酶对五氯联苯降解的初步研究
引用本文:生物酶对五氯联苯降解的初步研究. 生物酶对五氯联苯降解的初步研究[J]. 山东科学, 2016, 29(6): 94-97. DOI: 10.3976/j.issn.1002-4026.2016.06.015
作者姓名:生物酶对五氯联苯降解的初步研究
作者单位:山东省科学院生态研究所, 山东省应用微生物重点实验室, 山东 济南 250014
基金项目:国家国际科技合作专项(2014DFE90100);山东省自然科学基金(ZR2015YL008, BS2015HZ011)
摘    要:多氯联苯(PCBs)是具有代表性的持久性有机污染物(POPs),其氯代数目越多,降解越难。本文以分离筛选的6株PCBs降解细菌为材料,制备了含有NADH辅酶再生系统关键酶甲酸脱氢酶(FDH)的PCBs降解复合酶,研究了其对PCBs的降解效果。结果表明,Pseudomonas sp. MGB11胞内酶/FDH复合酶对五氯联苯PCB114的降解率在10 h内可达69.4%,对于高毒性、难降解的高氯PCBs的污染修复具有应用价值。

关 键 词:降解复合酶  五氯联苯   甲酸脱氢酶  
收稿时间:2016-08-08

Pilot study on bioenzyme degradation to pentachlorodiphenyl
JI Lei,CHEN Guan-hong,FU Xiao-wen,HUAGN Yu-jie,WANG Jia-ning,ZHANG Qiang. Pilot study on bioenzyme degradation to pentachlorodiphenyl[J]. Shandong Science, 2016, 29(6): 94-97. DOI: 10.3976/j.issn.1002-4026.2016.06.015
Authors:JI Lei  CHEN Guan-hong  FU Xiao-wen  HUAGN Yu-jie  WANG Jia-ning  ZHANG Qiang
Affiliation:Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Shandong Academy of Sciences, Jinan 250014, China
Abstract:Polychlorinated biphenyls (PCBs) is a representative of persistent organic pollutants (POPs). More its chlorinated number leads to more difficult its degradation . We prepared PCBs compound degrading enzyme of key enzyme formate dehydrogenase (FDH) of NADH coenzyme regeneration system with isolated 6 PCBs degradation bacteria strains as materials. We address its degrading effect to PCBs. Results indicate that degradation rate of Pseudomonas sp. MGB11/FDH compound enzyme to pentachlorodiphenyl PCB114 is up to 69.4% within 10 h. It therefore has application value in the repair for high chlorine PCBs pollutants with high toxicity and low degradability.
Keywords:pentachlorodiphenyl  formate dehydrogenase  compound degrading enzyme  
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