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霜霉病高效生防菌株BCJB01发酵培养基的优化
引用本文:霜霉病高效生防菌株BCJB发酵培养基的优化. 霜霉病高效生防菌株BCJB01发酵培养基的优化[J]. 山东科学, 2016, 29(4): 30-34. DOI: 10.3976/j.issn.1002-4026.2016.04.007
作者姓名:霜霉病高效生防菌株BCJB发酵培养基的优化
作者单位:山东省科学院生物研究所,山东省应用微生物重点实验室,山东 济南 250014
基金项目:山东省重点研发计划(2015GNC113002);山东省科学院-枣庄市产业技术研发联合基金(201503);公益性行业( 农业) 科研专项(201203035)
摘    要:为提高霜霉病高效生防菌株Bacillus cereus BCJB01的发酵水平,通过正交试验、综合优选及糖分流加试验,优化BCJB01的发酵培养基。优化出的培养基配方的质量分数为:玉米粉2.00%、豆粕粉1.00%、四水氯化锰0.003 0%、十二水磷酸氢二钠0.30%、二水磷酸二氢钠0.15%、七水硫酸镁0.035%、氯化钙0.10%和酵母提取物0.30%,接菌后6~12 h流加质量分数0.2%糖。本研究优化的培养基配方在接种量为3%(V/V),转速为180 r /min,温度为 30 ℃的摇床振荡培养至66 h时,芽孢率近90%,菌体数量达100×108 cfu/mL以上。

关 键 词:蜡样芽孢杆菌  正交设计  综合优选  霜霉病  发酵培养基  
收稿时间:2016-04-27

Optimization of fermentative medium for highly effective biocontrol strainBacillus cereus BCJB01 against downy mildew
WANG Yi-lian,XIE Xue-ying,HU Jin-dong,CHEN Kai,YANG He-Tong,LI Ji-shun. Optimization of fermentative medium for highly effective biocontrol strainBacillus cereus BCJB01 against downy mildew[J]. Shandong Science, 2016, 29(4): 30-34. DOI: 10.3976/j.issn.1002-4026.2016.04.007
Authors:WANG Yi-lian  XIE Xue-ying  HU Jin-dong  CHEN Kai  YANG He-Tong  LI Ji-shun
Affiliation:Shandong Provincial Key Laboratory of Applied Microbiology, Biology Institute, Shandong Academy of Sciences, Jinan 250014, China
Abstract:To improve fermentation level of Bacillus cereus BCJB01, a highly efficient biocontrol strain against downy mildew, we optimized its fermentative medium through orthogonal test, comprehensive optimization and sugar feeding tests. Mass fraction of the optimized medium formula are 2.00% corn meal,1.00% soybean powder, 0.0030% manganese chloride tetrahydrate, 0.30% twelve aqueous disodium hydrogen phosphate, 0.15% sodium dihydrogen phosphate dihydrate, 0.035% magnesium sulfate heptahydrate, 0.10% calcium chloride, 0.30% yeast extract, 0.2%sugar added at 6~12 h after inoculation. It can produce more than 100×108 cfu/mL cells and its spore rate is 90%, when the cultivation conditions are 3%(V/V) inoculum size, 180 r / min rotation velocity, 30 ℃ shaky cultivation till 66 hours.
Keywords:orthogonal design  downy mildew  Bacillus cereus  fermentative medium  comprehensive optimization  
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