Cytochrome P450 Monooxygenase Immobilization as a Model of Herbicide Metabolism in vitro |
| |
| Authors: | Xiang Wensheng Wang Xiangjing Ju Xiulian Ren Tianrui |
| |
| Institution: | Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, P.R.China;Research Center of Life Science and Biotechnology, Northeast Agricultural University, Harbin 150030, P.R.China;Research Center of Life Science and Biotechnology, Northeast Agricultural University, Harbin 150030, P.R.China;Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, P.R.China |
| |
| Abstract: | To investigate herbicide metabolism in vitro by cytochrome P450 with stable enzymatic activity, cytochrome P450 is immobilized in silk fibroin. The enzymatic activity of immobilized cytochrome P450 is maintained above 80% after repeated batch experiments for 10 times. Moreover, the enzymatic activity of immobilized cytochrome P450 is kept as relatively high as 73.8% after storage for two months at 4℃. In addition, immobilization can improve the temperature and pH stability of cytochrome P450. Immobilized cytochrome P450 has the similar affinity Km values for herbicide chlorsulfuron and triasulfuron as the free cytochrome P450. In the case of chlorosulfuron, affinity Km value is 53μmol/L for free cytochrome P450, and 63μmol/L for immobilized cytochrome P450, respectively. In the case of triasulfuron affinity, Km value is 36μmol/L for free cytochrome P450, and 44μmol/L for immobilized cytochrome P450, respectivily. Immobilized cytochrome P450 will be convenient, rapid, stable and continuous for herbicide metabolism in micro-bioreactor in vitro. |
| |
| Keywords: | cytochrome P450 immobilization herbicide metabolism in vitro |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
