| 瑞香狼毒对东亚飞蝗作用机理的初步研究 |
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| 引用本文： | 陈琼,高甜甜,李波,查艳梅,陶科,金洪,侯太平. 瑞香狼毒对东亚飞蝗作用机理的初步研究[J]. 四川大学学报(自然科学版), 2017, 54(3): 665-669 |
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| 作者姓名： | 陈琼 高甜甜 李波 查艳梅 陶科 金洪 侯太平 |
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| 作者单位： | 四川大学生命科学学院生物资源与生态环境教育部重点实验室,,,,,,四川大学生命科学学院生物资源与生态环境教育部重点实验室 |
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| 基金项目： | 国家自然科学基金(31272068) ；四川省应用基础研究项目（2014JY0063） |
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| 摘 要： | 利用硅胶柱分离瑞香狼毒乙酸乙酯萃取物得到Ⅰ、Ⅱ、Ⅲ段粗分物,并测定对东亚飞蝗毒杀活性最高的I段粗分物对东亚飞蝗的致死性及对其体内Na~+-K~+-ATPase、Ca~(2+)/Mg~(2+)-ATPase、乙酰胆碱酯酶的抑制活性.研究表明:瑞香狼毒I段粗分物对东亚飞蝗具有较强的毒杀作用,在药物浓度为10mg/mL、72h对东亚飞蝗的校正死亡率为92.50%;同时,毒杀作用具有浓度和时间依赖性;而且瑞香狼毒乙酸乙酯萃取物I段粗分物对上述3种酶均有一定的抑制作用,且对Na~+-K~+-ATPase的抑制作用最强,在药物浓度为5mg/mL、96h对Na~+-K~+-ATPase的抑制率为40.38%;分析发现瑞香狼毒活性成分对东亚飞蝗的毒杀作用与对Na~+-K~+-ATPase的抑制作用之间具有显著相关性,据此推测,Na~+-K~+-ATPase可能是瑞香狼毒活性成分的主要作用靶标之一.
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| 关 键 词： | 瑞香狼毒；东亚飞蝗；Na+-K+-ATPase；Ca2+/Mg2+-ATPase；乙酰胆碱酯酶 |
| 收稿时间： | 2016-03-16 |
| 修稿时间： | 2016-04-29 |
Studies on the mechanism of Stellera chamaejasme L. to Locusta migratoria manilensis (Meyen) |
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| CHEN Qiong,GIAO Tian-Tian,LI Bo,ZHA Yan-Mei,TAO Ke,JIN Hong and HOU Tai-Ping. Studies on the mechanism of Stellera chamaejasme L. to Locusta migratoria manilensis (Meyen)[J]. Journal of Sichuan University (Natural Science Edition), 2017, 54(3): 665-669 |
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| Authors: | CHEN Qiong GIAO Tian-Tian LI Bo ZHA Yan-Mei TAO Ke JIN Hong HOU Tai-Ping |
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| Affiliation: | Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University,Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University,Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University,Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University,Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University,Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University and Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University |
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| Abstract: | |
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| Keywords: | Stellera chamaejasme L. Locusta migratoria manilensis (Meyen) Na+-K+-ATPase Ca2+/Mg2+-ATPase acetylcholinesterase |
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