| 330弱碱性树脂固定化漆酶的酶学性质研究 |
| |
| 引用本文： | 王滨松,吕胜,张杰,杨士林,曹尔新.330弱碱性树脂固定化漆酶的酶学性质研究[J].黑龙江大学自然科学学报,2012,29(1):117-120,129. |
| |
| 作者姓名： | 王滨松 吕胜 张杰 杨士林 曹尔新 |
| |
| 作者单位： | 1. 黑龙江大学化学化工与材料学院,哈尔滨,150080
2. 东北林业大学生命科学学院,哈尔滨,150040 |
| |
| 基金项目： | 黑龙江省教育厅科学技术面上项目(11531270,12513038) |
| |
| 摘 要： | 为进一步拓展漆酶在污染物处理领域中的应用,通过330弱碱性树脂作为固定化载体,用戊二醛交联固定化漆酶,并对树脂固定化漆酶的酶学性质进行研究,研究结果表明:固定化漆酶在60℃体现了最佳活性,对温度的承受能力有所提高;固定化漆酶的最适pH在pH=3处,更耐酸碱性;固定化漆酶在放置30 d后,相对于初始酶活仍然保持了50%左右的酶活,循环使用10次后,相对酶活仍能保持初始酶活的45%左右。
|
| 关 键 词： | 漆酶 固定化 酶学性质 |
Study of laccase immobilization on 330 weakly alkaline resin and its performance |
| |
| WANG Bin-song
,
L Sheng
,
ZHANG Jie
,
YANG Shi-lin
,
CAO Er-xin.Study of laccase immobilization on 330 weakly alkaline resin and its performance[J].Journal of Natural Science of Heilongjiang University,2012,29(1):117-120,129. |
| |
| Authors: | WANG Bin-song L Sheng ZHANG Jie YANG Shi-lin CAO Er-xin |
| |
| Institution: | WANG Bin-song
,
L(U) Sheng
,
ZHANG Jie
,
YANG Shi-lin
,
CAO Er-xin |
| |
| Abstract: | Laccase has the ability to catalyze the degradation of certain pollutants,but because of it′s poor stability,high sensitivity to the surrounding environment and hard to reuse,the application of laccase in the field of pollutants treatment is limited.The laccase immobilization on 330 resins and its performance was investigated.The optimized pH value and temperature were pH=3 and 60 ℃,the pH stability and temperature stability increased.After keeping 30 days,the results indicated that reclaimed activity was 50%,and after 10 times’ use it still kept 45%. |
| |
| Keywords: | laccase immobilization enzymology character |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
