| 导入额外拷贝dctA基因的根瘤菌工程菌株的构建 |
| |
| 引用本文： | 蒙明明,于冰,李珊珊,李海英. 导入额外拷贝dctA基因的根瘤菌工程菌株的构建[J]. 黑龙江大学自然科学学报, 2012, 29(3): 391-394,400 |
| |
| 作者姓名： | 蒙明明 于冰 李珊珊 李海英 |
| |
| 作者单位： | 黑龙江大学生命科学学院,黑龙江省普通高等学校分子生物学重点实验室,哈尔滨150080;黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨150500 |
| |
| 基金项目： | 黑龙江省科技攻关资助项目，哈尔滨市科技创新人才研究专项资金资助项目，黑龙江大学高层次人才(创新团队)支持计划资助项目 |
| |
| 摘 要： | 为进一步提高大豆根瘤菌的固氮能力,以费氏中华根瘤菌(Sinorhizobium fredii)15067基因组的DNA作为模板,采用PCR扩增技术,克隆四碳二羧酸转移酶结构基因dctA的全长序列;将其连入lac启动子下游,构建带有LuxAB基因标记的pTR-Plac-dctA重组表达载体。利用三亲本杂交技术将表达载体转入费氏中华根瘤菌中,构建大豆根瘤菌工程菌株。研究表明,转基因工程菌株侵染大豆幼苗后与出发菌相比,固氮酶活性有了显著提高,为提高大豆产量的研究提供一定参考。
|
| 关 键 词： | dctA基因 转基因工程菌株 三亲本接合转移 |
Construction of genetic engineering strain of introduction extra dctA gene into rhizobium |
| |
| MENG Ming-ming , YU Bing , LI Shan-shan , LI Hai-ying. Construction of genetic engineering strain of introduction extra dctA gene into rhizobium[J]. Journal of Natural Science of Heilongjiang University, 2012, 29(3): 391-394,400 |
| |
| Authors: | MENG Ming-ming YU Bing LI Shan-shan LI Hai-ying |
| |
| Affiliation: | 1,2 (1.Key Laboratory of Molecular Biology,College of Heilongjiang Province,College of Life Sciences,Heilongjiang University,Harbin 150080,China;2.Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education,Heilongjiang University,Harbin 150500,China) |
| |
| Abstract: | In order to promote the ability of nitrogen fixation,the dctA gene copies in rhizobium fredii were increased.PCR technology was used to clone dicarboxylate transferase gene dctA with template of genomic DNA from Sinorhizobium fredii 15067.dctA gene was joined into downstream of lac promoter.And expression vector pTR-Plac-dctA was constructed by using plasmid pTR102 marked by luxAB gene.The expression vector pTR-Plac-dctA was transform into Sinorhizobium fredii 15067 with method of triparental hybridization to construct genetic engineering strain.The results showed that the nitrogen fixation ability of genetic engineering strain infect soybean seedlings more increase obviously than original strain.It will lay a foundation for the study of genetic engineering strain influence on nitrogen fixation ability of soybean. |
| |
| Keywords: | dctA gene genetic engineering strain triparental hybridization |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
