| 微型DNA条形码在鱼类识别上的应用 |
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| 引用本文： | 程鹏,张爱兵,王忠锁.微型DNA条形码在鱼类识别上的应用[J].首都师范大学学报(自然科学版),2012,33(2):47-52. |
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| 作者姓名： | 程鹏 张爱兵 王忠锁 |
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| 作者单位： | 首都师范大学生命科学学院,北京,100048 |
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| 基金项目： | 国家自然基金资助(Nr.31071963) |
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| 摘 要： | 本研究选用取自怀柔地区的53头鱼类样品作为实验材料,在经典的形态分类基础上,将其DNA条形码分类与微型DNA条形码分类效果进行比较,探讨微型条码技术在鱼类分类鉴定中的效果.将实验得到的COI序列均分为3段,对全条形码和不同的微型条码构建的进化树（NJ）与形态鉴定结果进行比较,得出全条形码鉴定与形态鉴定基本一致,同时发现了形态鉴定中的2处失误;3段不同微型条码的进化树表明,靠近5’端微型条码与DNA条形码分类结果基本一致,另外两段则效果不佳.说明适当的DNA微型条码可用于鱼类的鉴定,这将有利于鱼类的开发和保护.
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| 关 键 词： | 鱼类分类 生物入侵 DNA条形码 DNA微型条码 |
Mini DNA Barcode Utilizes in Fish Classification |
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| Peng Cheng
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Zhang Aibing
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Wang Zhongsuo.Mini DNA Barcode Utilizes in Fish Classification[J].Journal of Capital Normal University(Natural Science Edition),2012,33(2):47-52. |
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| Authors: | Peng Cheng Zhang Aibing Wang Zhongsuo |
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| Institution: | Peng Cheng Zhang Aibing Wang Zhongsuo(College of Life Science,Capital Normal University,Beijing 100043) |
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| Abstract: | We got 53 COI sequences of fish from Huairou area of Beijing.Combined with classification,the data from minimalist DNA barcode and DNA barcode were compared to testify if the minimalist DNA barcode was available for species identification.We divided 53 COI sequences into 3 segments,calculated the accuracy of these segments by carrying out neighbor-joining(NJ)analysis and analyzing the genetic distance.The results indicated that molecular identification consistent with the morphological identification is probably,and found two errors in classification.The accuracy of the first 200 bp barcode in species identification was consistent with the result of full COI.So it can be concluded that the identification of fish species could be done by minimalist DNA barcode.It will benefit the development and protection of fish. |
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| Keywords: | fish classification biological invasion DNA barcode mini barcode |
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