| 芦荟的组织培养 |
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| 引用本文： | 黄衡宇,李鹂,杨胜辉.芦荟的组织培养[J].吉首大学学报(自然科学版),2000,21(3):11-13. |
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| 作者姓名： | 黄衡宇 李鹂 杨胜辉 |
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| 作者单位： | (吉首大学生命科学与化学学院, 湖南 吉首 416000) |
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| 摘 要： | 针对芦荟自然繁殖率低下的情况, 探讨了利用组织培养方法解决生产中繁殖的问题.在叶和茎 2 种外植体中,嫩茎的出愈率最高,是理想的快速繁殖材料,较适宜的诱导愈伤组织的激素组合是 BA 2. 0 mg?L- 1+ IBA 0. 2 mg?L- 1, 诱导不定芽的适宜激素组合是 BA 3. 0 mg?L- 1+ NAA 1. 0 mg?L- 1, 而根的诱导则是在1/ 2MS+NAA 1. 0 mg?L- 1+ 庶糖 1. 5%+活性碳 3%培养基上进行.
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| 关 键 词： | 芦荟 组织培养 愈伤组织 不定芽 |
| 文章编号： | 1007-2985(2000)03-0011-03 |
| 修稿时间： | 2000年6月3日 |
Tissue Culture of Aloe vera L. |
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| HUANG Heng-Yu,LI Li,YANG Sheng-Hui.Tissue Culture of Aloe vera L.[J].Journal of Jishou University(Natural Science Edition),2000,21(3):11-13. |
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| Authors: | HUANG Heng-Yu LI Li YANG Sheng-Hui |
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| Institution: | ( The Biology Department of Jishou University, Jishou 416000 , Hunan China) |
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| Abstract: | Because the fruiting rate and germinative percentage of Aloe vera L. are lower, tissue culture was explored to solve the propagating problem in the production. The results show that the bud is the best material in speeding propagation among the two explants ( stem, leaf ) as it has the biggest inductivity, the best growth and it is easy to be in ducted adventitious buds. The suitable phytohormone compositions to induct callus, adventitious buds and roots are respectively BA 2. 0 mg?L- 1+ IBA 0. 2 mg?L- 1, BA 3. 0 mg?L- 1+ NAA 1. 0 mg?L- 1, 1/ 2 MS+ NAA 1. 0 mg?L- 1+ sugar 1. 5% + carbon3%. |
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| Keywords: | Aloe vera L Aloe vera L" target="_blank">')" href="#">Aloe vera L tissue culture callus adventitious bud |
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