A simple test using restricted PCR-amplified mitochondrial DNA to study the genetic structure ofApis mellifera L |
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Authors: | L Garnery M Solignac G Celebrano J -M Cornuet |
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Institution: | (1) Laboratoire de Neurobiologie Comparée des Invertébrés, INRA-CNRS URA 1190, La Guyonnerie, B.P. 23, F-91440 Bures-sur-Yvette, France;(2) Laboratoire de Biologie et Génétique Evolutives, CNRS, Avenue de la Terrasse, F-91198 Gif-sur-Yvette, France;(3) Dipartmmento di Biologie Animale, Università di Torino, Via Accademia Albertina 17, I-10123 Torino, Italy |
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Abstract: | The COI-COII intergenic region ofApis mellifera mitochondrial DNA contains an important length polymorphism based on a variable number of copies of a 192–196 bp sequence (Q) and the completer or partial deletion of 67 pb sequence (Po). This length variability has been combined with a restriction site polymorphism to produce a rapid and simple test for the characterization of mtDNA haplotypes. This test included the amplification by the polymerase chain reaction of the COI-COII region followed by aDraI restriction of the amplified fragment. In a survey of 302 colonies belonging to 12 subspecies, 21 different haplotypes have been found which have been unambiguously allocated to one of the 3 mtDNA lineages of the species. Although all colonies of lineage C exhibit the same pattern (C1), each one of lineages A and M presents up to 10 different haplotypes, opening the way to studies on the genetic structure and the evolution of a large fraction of the species. This test also differentiates southern Spanish and South African colonies, which can be of great interest for the Africanized bee problem. |
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Keywords: | Apis mellifera honeybee mitochondrial DNA restriction fragment length polymorphism DraI polymerase chain reaction COI-COII intergenic region |
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