Comparative proteomic analysis of the response in resistant and susceptible maize inbred lines to infection by Curvularia lunata |
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Authors: | Xiuli Huang Lixing Liu Jie Chen Yuhong Zhai |
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Institution: | Department of Plant Science, School of Agriculture and Biology, Key Laboratory of Microbial Metabolism, Ministry of Education, Shanghai Jiaotong University, Shanghai 200240, China |
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Abstract: | Proteins differentially expressed from maize leaves in response to the infection by Curvularia lunata strain CX-3 were identified through a high-resolution two-dimensional gel electrophoresis (2-DE) method. Two inbred lines, 78599-1 and E28, were used, respectively, as resistant and susceptible lines to CX-3 infection. Proteins were extracted from the fourth leaves of six- or seven-leaf stage plants sampled at 24, 36, 48, 60, and 72 h after inoculation with CX-3. Twenty-seven differentially expressed protein spots resolved on the 2-DE gels were identified by MALDI-TOF MS/MS. The results showed that these proteins are associated with photosynthesis, respiration,oxidative and drought stress tolerance as well as signal transduction in maize. Among stress-related proteins, the 22 kDa drought-inducible protein, putative glutathione peroxidase (GPX), and translation initiation factor (eIF-5A) were up-regulated in the resistant inbred line and were implicated in host defense response to C. lunata infection. It suggests that drought-inducible and oxidation stress-related proteins might directly contribute to maize resistance to C. lunata. |
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Keywords: | Curvularia lunata Maize Proteomies Two-dimensional electrophoresis (2-DE) |
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