| 黄瓜子叶节离体再生体系的建立 |
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| 引用本文： | 崔波,梁芳,程喜梅,袁丽洁,叶永忠.黄瓜子叶节离体再生体系的建立[J].河南科学,2008,26(3):288-290. |
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| 作者姓名： | 崔波 梁芳 程喜梅 袁丽洁 叶永忠 |
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| 作者单位： | 1. 河南农业大学,生命科学学院,郑州,450002;郑州师范高等专科学校,生物技术研究所,郑州,450044
2. 河南农业大学,生命科学学院,郑州,450002 |
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| 基金项目： | 河南省郑州市科技攻关计划 |
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| 摘 要： | 以MS为基本培养基,以黄瓜子叶节为外植体,设置了不同激素浓度,直接诱导出不定芽,建立了黄瓜的离体再生体系.结果表明,最佳芽诱导培养基为MS+BA 1.0 mg/L+IAA 0.1 mg/L+ABA 1.0 mg/L+AgNO3 2.0 mg/L,平均每外植体可诱导出2.9个芽;最佳芽伸长培养基为MS+KT 0.5 mg/L;1/2 MS最适于黄瓜的生根.
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| 关 键 词： | 黄瓜 子叶节 不定芽 再生体系 |
| 文章编号： | 1004-3918(2008)03-0288-03 |
| 修稿时间： | 2007年12月16 |
The Establishment of Regeneration System in Vitro from Cotyledonary Nodes of Cucumis sativus L. |
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| Cui Bo,Liang Fang,Cheng Ximei,Yuan Lijie,Ye Yongzhong.The Establishment of Regeneration System in Vitro from Cotyledonary Nodes of Cucumis sativus L.[J].Henan Science,2008,26(3):288-290. |
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| Authors: | Cui Bo Liang Fang Cheng Ximei Yuan Lijie Ye Yongzhong |
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| Institution: | Cui Bo1,2,Liang Fang1,Cheng Ximei1,Yuan Lijie1,Ye Yongzhong1(1.College of Life Science,Henan Agricultural University,Zhengzhou 450002,China,2.Institute of Biotechnology,Zhengzhou Teacher\'s College,Zhengzhou 450044,China) |
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| Abstract: | In this paper,the regeneration system in vitro of Cucumis sativus L.was established by using the cotyledonary nodes as explant.The results show that the medium of MS + BA 1.0 mg/L+IAA 0.1 mg/L +ABA 1.0 mg/L+AgNO3 2.0 mg/L is suitable for adventitious shoots inducing.In this medium averagely 2.9 shoots can be induced each explant.The best shoot elongation medium is MS+KT 0.5 mg/L and 1/2 MS is optimum for rooting of regenerative plant. |
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| Keywords: | Cucumis sativus L cotyledonary node adventitious shoot regeneration system |
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