微生物转化法生成橙皮素的研究

用以橙皮苷为转化底物和主要碳源的培养基筛选到能高效转化橙皮苷生成橙皮素的青霉菌C-21.并利用"复杂系统状态定向调控技术"对种子培养和发酵条件进行优化,使发酵时间缩短至58 h,转化率达到99.55%.根据茵种的动力学曲线发现优化后的培养条件可以提前起始产酶时间,增强产酶能力.

Biotransformation of hesperidin to hesperetin by microorganisms

A filamentous fungi strain, Penicillium C-21, producing/9-glucosidase was screened to effi-ciently transform hesperidin into hesperetin by using the medium whose substrate and main carbon source was hesperidin. Using the "Directional regulation technique of complex system" to modulate the fermentation's temperature, carbon source, nitrogen source, inorganic salt, rotary speed and medium capacity, etc, the condition of seed culture and fermentation could be optimized. Through analysis the strain's fermentation dynamics curve, it was found that cultivating strain in this condition could extended the time transforming hesperidin into hesperetin decurtated to 58 hours, and the bioconversion rate could reach 99.55%.