不同部位灵芝及子实体提取物中总三萜酸含量测定

建立不同部位灵芝及子实体提取物中总三萜酸含量测定方法.灵芝样品用乙醇提取,依次经氯仿、5%碳酸氢钠萃取,酸化后再用氯仿萃取、浓缩至干,称重,即得灵芝总三萜酸量.结果:灵芝子实体总三萜酸为(8.58±0.25)mg/g,孢子粉为(3.48±0.03)mg/g,菌丝体仅为(1.75±0.09)mg/g;菌盖总三萜酸含量为(12.62±0.22)mg/g,菌柄为(7.66±0.08)mg/g.10批次灵芝子实体的总三萜酸含量范围为4.3416.39 mg/g.灵芝子实体提取物中的总三萜酸含量以醇-水提取物的最高为(208.70±5.54)mg/g;碱水提取物的最低,仅为(123.07±4.99)mg/g.HPLC初步分析子实体和孢子及子实体提取物间三萜酸成分大部分相同.本法适用于灵芝子实体及其提取物、灵芝孢子和菌丝体总三萜酸的含量测定,结果可靠,可为灵芝产品质量指标提供依据.

Determination of total triterpenoid acids in different part and extract of Ganoderma lucidum

Aim To develop a method for determination of total triterpenoid acids in different part and extracts of Ganoderma lucid- urn. Method The samples of Ganoderma lucidum were extracted with ethanol and successively extracted with CHC13 and 5% Nail- CO3 , the NaHCO3 layer was acidified to pH 3 with 2 mol/L HC1, the resulting precipitates were dissolved in CHC13 and evaporated in vaeuo then weighed. The total triterpenoid acids were obtained. Result The total triterpenoid acids of Ganoderma lucidum fruit- body, spore and mycelium were （ 8.58 ~ 0.25 ） mg,/g, （ 3.48 ± 0.03 ） mg/g and （ 1.75 ± 0.09 ） mg/g respectively. The total triterpenoid acids of pileus and stipe were （ 12.62 ±0.22） mg/g and （7.66 ±0. 08） mg/g. The range of total triterpenoid acid content among 10 batches of C, anoderrna lucidum fruitbody purchased from the market was between 4.34 to 16.39 mg/g. The highest content fro/8/8/88/m Ganoderma lucidum fruiting body with alcohol - water extracting was （ 208.70 ~ 5.54 ） mgc/g and the lowest content with alkaline solution extracting was （ 123.07 ：t 4.99 ） mg/g. The composition of total triterpenoid acid from fruitbody, spores and extract of fruitbody analyzed by HPLC were almost the same. This method is reliable for determination of total triterpenoid acid in the fruiting body and its extracts, spore and mycelium from Ganoderma lucidum,which provides an in- dicator for the quality of Ganoderma lucidum product.