Strategies for cloning unknown cellular flanking DNA sequences from foreign integrants |
| |
| Authors: | E. K.-W. Hui P.-C. Wang S. J. Lo |
| |
| Affiliation: | (1) Institute of Microbiology and Immunology, School of Life Science, National Yang-Ming University, Taipei 11221 (Taiwan), Fax +886 2 28212880, e-mail: losj@ym.edu.tw, TW;(2) Department of Medicine, School of Medicine, National Yang-Ming University (Taiwan), TW |
| |
| Abstract: | Many virus and transposon DNAs can integrate into the host genome. In this review, techniques, including inverse polymerase chain reaction (IPCR), novel Alu-PCR and vectorette- or splinkerette-PCR are introduced as possible strategies for cloning flanking DNA regions of the integrants. Targeted gene-walking PCR, restriction-site PCR, capture PCR, and panhandle PCR and boomerang DNA amplification are also described. The principles, advantages and limitations of each approach are discussed. Received 9 July 1998; received after revision 2 October 1998; accepted 7 October 1998 |
| |
| Keywords: | . Boomerang DNA amplification (BDA) capture PCR (CPCR) inverse PCR (IPCR) novel Alu-PCR panhandle PCR polymerase chain reaction (PCR) restriction-site PCR (RS-PCR) splinkerette-PCR targeted gene-walking PCR vectorette-PCR. |
| 本文献已被 SpringerLink 等数据库收录！ |
|
