超滤质谱法研究人血清白蛋白与甘草提取物的相互作用

为研究和筛选甘草提取物中的活性成分,采用超滤质谱法(UF-MS)研究了人血清白蛋白和5种甘草提取物(甘草苷、甘草酸、甘草素、甘草呋喃酮和甘草利酮)的相互作用.结果表明:这5种甘草提取物均与人血清白蛋白有不同程度的结合,其中甘草酸的结合能力最强,其次是甘草苷、甘草素、甘草呋喃酮和甘草利酮.说明超滤质谱法可准确识别与受体蛋白质结合的活性小分子配体,实现天然产物活性成分的快速筛选.

Study on the Interaction between Human Serum Albumin and Licorice Extracts by Ultrafiltration Mass Spectrometry

In order to study and screen the active ingredient in licorice extract, the interaction between human serum albumin and 5 licorice extracts (liquorice glycosides, glycyrrhizic acid, licorice, licorice furfuran ketone and licoricone) were studied using ultrafiltration mass spectrometry (UF-MS). The results showed that all of the 5 licorice extracts could bind with human serum albumin (HSA). Among them, glycyrrhizic acid had the highest binding affinity, followed by liquorice glycosides, licorice, licorice furfuran ketone and licoricone. Our work demonstrated that UF-MS could accurately identify the active small molecule ligands of the receptor protein, thus rapidly screen active ingredients from natural products.