| 根癌土壤农杆菌Ti质粒毒性区VirD1基因的克隆及序列分析 |
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| 引用本文： | 刘红玲,祝建波,朱新霞,楚敏.根癌土壤农杆菌Ti质粒毒性区VirD1基因的克隆及序列分析[J].石河子大学学报,2004,22(3):194-196. |
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| 作者姓名： | 刘红玲 祝建波 朱新霞 楚敏 |
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| 作者单位： | 石河子大学生物工程学院，石河子大学生物工程学院，石河子大学生物工程学院，石河子大学生物工程学院 新疆 石河子 832003，新疆 石河子 832003，新疆 石河子 832003，新疆 石河子 832003 |
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| 基金项目： | 新天石大科研基金(XS200009) |
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| 摘 要： | 根据U．Washington报导的根癌土壤农杆菌(Agribacterium tumefaciens)C58Ti质粒基因序列，设计1对引物，利用PCR的方法扩增了c58根癌土壤农杆菌Ti质粒毒性区VirD1基因。通过琼脂糖凝胶电泳，所得E1的片段略小于500bp，与引物设计跨幅片段476bp相吻合。将此片段连接到T载体，经蓝白斑筛选、PCR鉴定、测序及DNA序列分析，结果表明：克隆VirD1基因编码序列与报导的序列同源性达100％，说明通过PCR方法获得的VirD1基因片段是正确的，为进一步研究该基因的表达和活性奠定了基础。
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| 关 键 词： | VirD1基因 克隆 序列分析 |
| 文章编号： | 1007-7383(2004)03-0194-04 |
| 修稿时间： | 2003年11月3日 |
Cloning and Sequencing of the VirD 1 Protein Gene Situating in Virus Region of Agribacterium Tumefaciens' Ti Plasmid |
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| LIU Hong-ling,ZHU Jian-bo,ZHU Xin-xia.Cloning and Sequencing of the VirD 1 Protein Gene Situating in Virus Region of Agribacterium Tumefaciens'''' Ti Plasmid[J].Journal of Shihezi University(Natural Science),2004,22(3):194-196. |
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| Authors: | LIU Hong-ling ZHU Jian-bo ZHU Xin-xia |
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| Abstract: | According to the Agribacterium tumefaciens' Ti plasmid gene sequences published by U. Washington, we designed a pair of primers, amplified the VirD1 protein gene situating in virus region of agribacterium tumefaciens' Ti Plasmid with the method of PCR. Though agarose electrophoresis the cloning fragment is lower a little than 500bp, which identical with the design length (476bp). Join the frament with T-vector, through blue and white spot selection, sequencing and DNA sequence analysis, the result shows:there are 100% accordance between cloning VirD1 gene coden sequence and the published gene sequence. Conclusion:Through PCR method we get the correct VirD1 protein gene sequence, this will be helpful to study the gene's translation and activity. |
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| Keywords: | VirD1 gene clonging sequence analysis |
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