| 低能N+注入凤仙花的变异检测 |
| |
| 引用本文： | 张涛,王淑芳,褚仲杰,苏颖,张桂芳.低能N+注入凤仙花的变异检测[J].北京师范大学学报(自然科学版),2006,42(2):171-173. |
| |
| 作者姓名： | 张涛 王淑芳 褚仲杰 苏颖 张桂芳 |
| |
| 作者单位： | 北京师范大学低能核物理研究所;北京师范大学生命科学学院,100875,北京 |
| |
| 摘 要： | 以低能N 注入凤仙花(Impatiens balsaminaL)种子后获得了高茎、矮茎2个变异品系.对其进行过氧化物同工酶(POD)检测的结果表明,2个变异品系叶片的POD分子大小、表达强度和条带数目与对照相比都发生了一定变化.随机扩增多态性DNA(random amplified polymorphic DNA,RAPD)分析结果也表明变异品系DNA链的脱氧核苷酸序列发生了变异,从25个随机引物中筛选出了9个具有多态性.实验证明适当剂量的低能N 注入能够在基因水平引起凤仙花的变异,为花卉产业的发展提供了一种遗传育种手段.
|
| 关 键 词： | 离子注入 凤仙花 过氧化物酶(POD) 随机扩增多态性DNA |
| 收稿时间： | 2005-11-16 |
| 修稿时间： | 2005年11月16日 |
EXAMINATION OF MUTATED IMPATIENS BALSAMINA CAUSED BY INJECTION OF NITROGEN IONS |
| |
| Zhang Tao,Wang Shufang,Chu Zhongjie,Su Ying,hang Guifang.EXAMINATION OF MUTATED IMPATIENS BALSAMINA CAUSED BY INJECTION OF NITROGEN IONS[J].Journal of Beijing Normal University(Natural Science),2006,42(2):171-173. |
| |
| Authors: | Zhang Tao Wang Shufang Chu Zhongjie Su Ying hang Guifang |
| |
| Institution: | 1.Institute of Low Energy Nuclear Physles, Beijing Radiation Center; 2. College of Life Sciences;Bejing Normal University, 100875, Beijing, China |
| |
| Abstract: | After being treated with low energy N~( )implantation,the two kinds of mutated Impatiens balsamina,one is higher and another is lower,are obtained.By using isozymic method it indicates the peroxidase variance in the molecular size,expression and the number of bands between the mutations and normal kind.By using random primers,Genetic constitutions of mutations and normal kind are analyzed by RAPD(random amplified polymorphic DNA) technique,9 primers have polymorphic fragments between higher and normal plants,3 primers have polymorphic fragments between lower and normal plants.This result suggests that the injection of nitrogen ions can cause the mutation of plant and be used in flower industry. |
| |
| Keywords: | ion implantation Impatiens balsamina peroxidase random amplified polymorphic DNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
