A spectrophotometric method for the determination of 5-phosphoribosyl-1-pyrophosphate |
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| Authors: | C Salerno A Giacomello E Messina |
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| Institution: | (1) Institue of Biological Chemistry and Institute of Rheumatology, University of Rome, I-00185 Rome, Italy;(2) Center of Molecular Biology of Consiglio Nazionale delle Ricerche, I-00185 Rome, Italy |
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| Abstract: | Summary A new, sensitive, specific and simple spectrophotometric method for the determination of 5-phosphoribosyl-1-pyrophosphate (PRPP) is presented. PRPP is reacted with excess hypoxanthine in the presence of hypoxanthine-guanine phosphoribosyltransferase. At the end of the reaction, PRPP concentration is measured from the extent of conversion of hypoxanthine to inositate. The concentration of the purine base is determined spectrophotometrically in the presence of xanthine oxidase. |
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