| 携带p53基因重组腺病毒载体的构建 |
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| 引用本文： | 夏胜,叶芳,梅兴国,洪琦. 携带p53基因重组腺病毒载体的构建[J]. 华中科技大学学报(自然科学版), 2001, 29(Z1): 55-57 |
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| 作者姓名： | 夏胜 叶芳 梅兴国 洪琦 |
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| 作者单位： | 华中科技大学生命科学与技术学院 |
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| 摘 要： | 将pCMVp53重组转移载体经BamHI和NheI酶切,得到p53基因cDNA,然后将cDNA片段克隆到转移载体pCMV5GFP,使其受CMV5启动子的调控,获得pCMV5p53重组转移载体.用该线状重组转移载体与腺病毒右臂DNA经磷酸钙共转染293细胞获得重组腺病毒,经酶联免疫吸附法(ELISA)测定p53蛋白含量,证明外源p53基因在含重组腺病毒的293细胞中得到表达.
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| 关 键 词： | p53基因 重组腺病毒 转移载体 |
| 文章编号： | 1000-8616(2001)1-0055-03 |
| 修稿时间： | 2000-09-25 |
Construction of p53 Recombinant Adenovirus Vector |
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| Xia Sheng Ye Fang Mei Xingguo Hong Qi Master, College of Life Sci. , Tech.,HUST,Wuhan ,China.. Construction of p53 Recombinant Adenovirus Vector[J]. JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY.NATURE SCIENCE, 2001, 29(Z1): 55-57 |
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| Authors: | Xia Sheng Ye Fang Mei Xingguo Hong Qi Master College of Life Sci. & Tech. HUST Wuhan China. |
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| Affiliation: | Xia Sheng Ye Fang Mei Xingguo Hong Qi Master, College of Life Sci. & Tech.,HUST,Wuhan 430074,China. |
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| Abstract: | p53 cDNA is cloned into shuttle vector pCMV5GFP to get p53 recombinant adenovirus vector and make it controlled by CMV5 promotor and enhancer. And p53 cDNA is rescued into adenovirus by calcium phosphate cotransfection of linearized pCMV5p53 and QBI viral DNA into 293 cells and positive recombinant adenovirus plaques are screened by fluores centmicroscope. Expression of p53 gene in 293 cell is detected by EALISA. p53 recombinant adenovirus vector can be constructed and used for further research. |
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| Keywords: | p53 gene recombinant adenovirus shuttle vector |
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