| 酵母人工染色体DNA的大尺度物理图谱分析 |
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| 引用本文： | 柴建华 王乐. 酵母人工染色体DNA的大尺度物理图谱分析[J]. 复旦学报(自然科学版), 1993, 32(4): 394-398 |
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| 作者姓名： | 柴建华 王乐 |
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| 作者单位： | 复旦大学遗传学研究所，复旦大学遗传学研究所，复旦大学遗传学研究所，复旦大学遗传学研究所 1991届博士研究生，1991届博士研究生 |
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| 摘 要： | 以不同ＰＢＲ３２２质粒ＤＮＡ片段作为ＹＡＣＤＮＡ左右臂探针，使用稀切点限制性内切酶和脉冲电泳技术对含有２ｂＡ３的８００ｋｂ ＹＡＣ作了４种限制酶大尺度物理图谱分析。
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| 关 键 词： | YAC DNA 酵母 大尺度 物理图谱 |
LONG RANGE PHYSICAL MAP OF YAC DNA |
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| Chai jianhua,Gu Yanghong,Wang Le,Zhang Cheng. LONG RANGE PHYSICAL MAP OF YAC DNA[J]. Journal of Fudan University(Natural Science), 1993, 32(4): 394-398 |
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| Authors: | Chai jianhua Gu Yanghong Wang Le Zhang Cheng |
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| Affiliation: | Institute of Genetics |
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| Abstract: | Different fragments of plasmid pBR322 DNA were used as the left and right arms probes of YAC (Yeast Artificial Chromosome). The rare cutting restriction enzyme and pulsed field electrophoresis were used for long range physical mapping of YACs. A YAC containing 2bA3 DNA locus with the length of 800 kb was analysed with four different restriction enzyme to obtain the long range physical map. |
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| Keywords: | YAC pulsed field electrophoresis 2bA3 long range physiscal map |
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