| 何首乌特征图谱分析方法研究 |
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| 引用本文： | 杨鑫,何华,王建新,龙滢,林绍霞,林昌虎. 何首乌特征图谱分析方法研究[J]. 贵州科学, 2014, 0(3): 59-63,85 |
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| 作者姓名： | 杨鑫 何华 王建新 龙滢 林绍霞 林昌虎 |
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| 作者单位： | [1] 黔东南州食品药品检验所,凯里556000 [2] 贵州省分析测试研究院,贵阳550002 [3] 贵州科学院,贵阳550001 |
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| 基金项目： | 贵州省中药现代化重大专项项目（黔科合重大专项字[2012]6010号）;贵州省国际科技计划项目（黔科合外G字[2013]7034号）. |
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| 摘 要： | 本文阐述了建立何首乌药材色谱定性分析方法。采用HPLC测定法,色谱柱phnomenex C18,4.6×250 mm,5μm;流动相:甲醇-水梯度洗脱;流速1.2 ml/min;检测波长280 nm;柱温40℃。对何首乌有效部位进行指纹图谱定性分析,从18个批次不同产地何首乌药材的指纹图谱中可检出4个相对位置稳定的色谱峰,作为定性鉴别的指标峰。利用何首乌药材指纹图谱可以作为对何首乌药材的定性分析和质量控制。
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| 关 键 词： | 何首乌 指纹图谱 高效液相色谱法 |
Study on Analysis of Characteristic Chromatogram of Polygonum |
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| YANG Xin,HE Hua,WANG Jian-xin,LONG Ying,LIN shao-xia,LIN Chang-hu. Study on Analysis of Characteristic Chromatogram of Polygonum[J]. Guizhou Science, 2014, 0(3): 59-63,85 |
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| Authors: | YANG Xin HE Hua WANG Jian-xin LONG Ying LIN shao-xia LIN Chang-hu |
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| Affiliation: | 1.Institute for Food and Drug of Southeast Guizhou, Kaili, Guizhou 556000, China ; 2. Guizhou Academy of Testing and Analysis, Guiyang , Guizhou 550002, China ; 3. Guizhou Academy of Sciences, Guiyang , Guizhou 550001, China) |
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| Abstract: | Objective： For the establishment of qualitative chromatographic analysis of polygonum. Method： Adoption of HPLC, chromatographic column phnomenex C 18,4. 6 ×250 mm,5μm ; mobile phase ： methanol-water gradient elution ; flow rate of 1.2 ml/min; detection wavelength of 280 nm ; 40℃ of column temperature. Result ： Qualitative analysis on fingerprint chromatogram for effective parts of polygonum, 4 relatively stable peak positions detected as peak indicators for qualitative identification of fingerprint chromatogram for 18 batches of polygonum herbs collected from different places of origin. Conclusion ： Fingerprint chromatogram of polygonum herb could be used for qualitative analysis and quality control. |
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| Keywords: | polygonum fingerprint chromatogram HPLC |
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