刚毛柽柳TheIF1A基因转入酵母的抗逆能力分析

翻译起始因子eIF1A基因是蛋白合成的重要调控因子之一,在一些植物中可能参与抗逆调控过程。为了研究刚毛柽柳TheIF1A基因是否参与抗逆过程,将TheIF1A基因插入酵母表达载体pYES2中构建成重组载体,转入酿酒酵母(Saccharomyces cerevisiae)中获得重组型酵母,分别比较转TheIF1A基因酵母和转空载体对照酵母在山梨醇、H₂O₂、CdCl₂、CuSO₄、ZnCl₂、KCl、Na₂CO₃、MgCl₂、-20 ℃和53 ℃胁迫处理之后的存活能力。结果显示,TheIF1A基因能有效提高转基因酵母的抗干旱、盐碱、氧化、重金属及极端温度的能力,表明TheIF1A可能参与了柽柳多种抗逆调控过程。

Stress tolerance analysis of a Tamarix hispida TheIF1A in Saccharomyces cerevisiae

Translation initiation factor 1A is an important regulation factor for protein synthesis, which may involve in plant abiotic stress regulation. In order to study the stress tolerance of a translation initiation factor 1A(TheIF1A) from Tamarix hispida, the recombinant plasmid, named as pYES2-TheIF1A, was constructed by inserting the TheIF1A gene into the yeast expression vector pYES2. The plasmid pYES2-TheIF1A was transformed into the yeast Saccharomyces cerevisiae, and the yeast transformed with empty pYES2 was employed as a control. The pYES2-TheIF1A and pYES2 recombinant yeast were respectively treated with sorbitol, H₂O₂, CdCl₂, CuSO₄, ZnCl₂, KCl, Na₂CO₃, MgCl₂, low temperature -20 ℃ and high temperature 53 ℃, respectively. Results showed that the yeast expressing TheIF1A had greater tolerant to salt, drought, heavy metals, and both low and high temperatures than the control, suggesting that TheIF1A gene might an important stress-regulating gene in T.hispida.