| 利用离子色谱测定海带硫酸多糖中硫酸根的含量 |
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| 引用本文： | 马心英.利用离子色谱测定海带硫酸多糖中硫酸根的含量[J].曲阜师范大学学报,2002,28(2):84-86. |
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| 作者姓名： | 马心英 |
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| 作者单位： | 菏泽师范专科学校化学系 274015，山东省菏泽市 |
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| 摘 要： | 建立了用离子色谱测定海带硫酸多糖硫酸根的方法。海带经酶解，季胺盐沉淀，离子交换和凝胶过滤，从中提取分离到3种多糖：F1，F2，F3，在对3个组分进行了GPC及红外光谱分析，证明匀为均一硫酸多糖的基础上，2组分分别经灰化，定容处理后，利用离子色谱对它们的硫酸根含量分别进行了测定，结果分别为：F1：28.41KD,F2:15.87KD,F3:32.50KD。与传统硫酸钡浊度法相比，该方法消除了其它离子及杂质的干扰，操作简单，具有精确，灵敏，高效等许多优点，是测定硫酸多糖硫酸根含量的理想方法。
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| 关 键 词： | 海带 离子色谱 硫酸根 硫酸多糖 |
| 文章编号： | 1001-5337(2002)02-0084-03 |
| 修稿时间： | 2001年11月19 |
DETERMINATION OF SULFATE GROUP OF SULFATED POLYSAACHARIDES FROM LAMINARIA BY ION-CHROMATOGRAPHY |
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| MA Xin-ying.DETERMINATION OF SULFATE GROUP OF SULFATED POLYSAACHARIDES FROM LAMINARIA BY ION-CHROMATOGRAPHY[J].Journal of Qufu Normal University(Natural Science),2002,28(2):84-86. |
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| Authors: | MA Xin-ying |
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| Abstract: | An ion-chromatographic method for the determination of sulfate group of sulfated posaccharidesfrom laminaria was described . Three polysaccharide fractions (F1 , F2 , F3) were separated from laminaria with enzymatic lysis precipitation by quaternary ammonium , ion exchange and gel filtration . After three fractions were shown to be homogenous sulfated polysaccharides by GPC and IR , the determination of sulfate group was performed following combustion by ion-chromatography respectively . The sulfated group content of F1 , F2 , F3 was 28.41% , 15.87% , 32.50% , respectively. Due to removing interference from other ions and impurities , the ion-chromatographic method was accurate , sensitive , quick and simple comparing with turbidimetry , so this is an ideal method for the determination of sulfate group of sulfated posaccharides . |
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| Keywords: | ion-chromatography sulfate group sulfated polysaccharide |
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