| A novel human gene spindlin1, encoding a protein localized in the cell nucleus and inducing NIH3T3 cell's transformation |
| |
| 作者姓名： | GAO Yanhong QIN Lipeng ZHANG Peng CHEN Lin YUAN Hongfeng BAI Cixian YAN Fang YUE Wen PEI Xuetao |
| |
| 作者单位： | Department of Stem Cell Biology, Beijng Institute of Transfusion Medicine, Beijing 100850, China,Department of Stem Cell Biology, Beijng Institute of Transfusion Medicine, Beijing 100850, China,Department of Stem Cell Biology, Beijng Institute of Transfusion Medicine, Beijing 100850, China,Department of Stem Cell Biology, Beijng Institute of Transfusion Medicine, Beijing 100850, China,Department of Stem Cell Biology, Beijng Institute of Transfusion Medicine, Beijing 100850, China,Department of Stem Cell Biology, Beijng Institute of Transfusion Medicine, Beijing 100850, China,Department of Stem Cell Biology, Beijng Institute of Transfusion Medicine, Beijing 100850, China,Department of Stem Cell Biology, Beijng Institute of Transfusion Medicine, Beijing 100850, China,Department of Stem Cell Biology, Beijng Institute of Transfusion Medicine, Beijing 100850, China |
| |
| 基金项目： | 国家自然科学基金，国家重点基础研究发展计划(973计划)，国家高技术研究发展计划(863计划) |
| |
| 摘 要： | A novel human gene, spindlin1, recently cloned in our laboratory, is highly expressed in the tissue of ovary cancer. To study its biological function, a vector expressing green fluorescent-spindlin1 fusion protein was constructed and transfected into COS-7 and NIH3T3 cells by lipofectamine methods. The results showed that the fusion protein pEGFP-N1-spindlin1 was localized in the nucleus of COS-7 and NIH3T3 cells. NIH3T3 cells which could stably express spindlin1 as a result of RT-PCR analysis compared with the parental NIH3T3 cells displayed a complete morphological change, improved the cell growth and increased the percentage of cells in G2/M phase (12.6% vs control cells at 3.4%). Furthermore, overexpressed spindlin1 cells formed colonies in soft agar, more motile in migration assay in vitro and formed tumors in nude mice. Our findings provide direct evidence that spindlin1 gene may be a prooncogene which is associated with tumorigenesis.
|
| 关 键 词： | spindlin1 gene transfection subcellular localization tumorigenesis |
A novel human gene spindlin1, encoding a protein localized in the cell nucleus and inducing NIH3T3 cell's transformation |
| |
| GAO Yanhong,QIN Lipeng,ZHANG Peng,CHEN Lin,YUAN Hongfeng,BAI Cixian,YAN Fang,YUE Wen,PEI Xuetao.A novel human gene spindlin1, encoding a protein localized in the cell nucleus and inducing NIH3T3 cell''s transformation[J].Progress in Natural Science,2004,14(11):966-970. |
| |
| Authors: | Gao Yanhong QIN Lipeng ZHANG Peng CHEN Lin YUAN Hongfeng BAI Cixian YAN Fang YUE Wen Pei Xuetao |
| |
| Institution: | Department of Stem Cell Biology, Beijng Institute of Transfusion Medicine, Beijing 100850, China |
| |
| Abstract: | A novel human gene, spindlin1, recently cloned in our laboratory, is highly expressed in the tissue of ovary cancer. To study its biological function, a vector expressing green fluorescent-spindlin1 fusion protein was constructed and transfected into COS-7 and NIH3T3 cells by lipofectamine methods. The results showed that the fusion protein pEGFP-N1-spindlin1 was localized in the nucleus of COS-7 and NIH3T3 cells. NIH3T3 cells which could stably express spindlin1 as a result of RT-PCR analysis compared with the parental NIH3T3 cells displayed a complete morphological change, improved the cell growth and increased the percentage of cells in G2/M phase (12.6% vs control cells at 3.4%). Furthermore, overexpressed spindlin1 cells formed colonies in soft agar, more motile in migration assay in vitro and formed tumors in nude mice. Our findings provide direct evidence that spindlin1 gene may be a prooncogene which is associated with tumorigenesis. |
| |
| Keywords: | spindlin1 gene transfection subcellular localization tumorigenesis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《自然科学进展(英文版)》浏览原始摘要信息 |
| 点击此处可从《自然科学进展(英文版)》下载免费的PDF全文 |
|
