人卵巢癌高转移细胞株裸鼠原位移植瘤模型

 卵巢癌仍是病死率最高的妇科恶性肿瘤。理想的动物模型对于卵巢癌生物学行为及治疗方法的探索等方面均有重要作用。肿瘤原位模型因具有相对较高的仿真性而成为动物实验研究的首选。本研究选用人卵巢癌高转移细胞株HO-8910PM作为成瘤细胞，拟建立裸鼠卵巢癌原位移植瘤模型，并对相关生物学特性进行研究。首先，将HO-8910PM细胞（细胞数约2×106）注射到雌性裸鼠皮下。待皮下成瘤后再通过显微外科手术将瘤体小块转移到其他裸鼠卵巢内，追踪观察原位肿瘤的生长和体内的转移情况，在荷瘤鼠处于濒死状态时将其处死，取原位和转移病灶处的肿瘤组织做光镜及电镜的病理检查。共14只裸鼠接受了原位移植手术，经追踪观察，有8只原位成瘤，成功率为57.14%。通过尸检发现有5只荷瘤鼠出现了远处器官的转移（转移率为83.33%），转移器官为肺、肝脏、膈肌、胃表面、肠管表面、胰脏、后腹膜、对侧卵巢、髂窝淋巴结等处，以膈肌和肝脏较为普遍。本研究成功地建立了裸鼠原位移植瘤模型，其生物学特性在某种程度上与人类卵巢癌更为相似，为卵巢肿瘤的研究提供了一种理想的动物模型。

An Orthotopic Ovarian Carcinoma Model with Metastasis in Nude Mice

Ovarian cancers are the leading cause of death among gynecologic tumors. Animal models are necessary for experimental cancer research and play an important role in the development of diagnostic and therapeutic modalities. The improvement of animal models to better simulate the conditions in patients is important for its role in the treatment for ovarian cancers. Orthotopic implantation is an attractive technique. Unlike heterotopic implantation, it allows a more accurate expression of the biologic nature of a human tumor, including growth, morphology and metastasis. The aim of the present study was to establish an orthotopic ovarian cancer model with high metastasis in nude mice. Human ovarian carcinoma cells HO-8910PM, a subline with highly metastatic potential, were inoculated subcutaneously in female nude mice to form solid tumors. The exuberantly growing tumor were removed, sliced and introduced into ovaries of other nude mice by microsurgical techniques. The growth and metastasis of the orthotopic lesion were followed, and pathological examinations were performed. Ovarian cancers formed in animals with an incidence of 57.14%. Metastatic lesions were detected in the lung, diaphragm, liver, stomach, intestine, pancreas, peritoneum, contralateral ovary and lymph nodes located in the iliac fossa, with an occurrence rate of 83.33% in animals. Transplanting tissues into the ovary of a nude mouse can form an orthotopic cancer model, and the manner how cancer spreads was similar to that of human lesions, which offers a remarkable model to explore ovarian cancers.