玉米大斑病菌HT-毒素特异性组分与原生质膜蛋白互作研究

以玉米叶片原生质为研究对象,以ANS(1-苯胺基-8-萘磺酸)作荧光探剂,用荧光标记法研究了毒素与质膜蛋白的互作.在被ANS标记后的原生质悬浮液中加入不同浓度HT-毒素后,ANS荧光强度明显增加,呈现出了剂量—效应关系,表明HT-毒素与质膜上ANS某些受体发生了互作.本实验采用2种试剂,从2个角度表明了ANS受体和质膜上存在的毒素受体具有蛋白特性.初步推测HT-毒素特异性组分的原初作用位点在质膜外侧.

Study of Interaction Between Specific Toxin Produced by Exserohilum Turcicum and Membrane Protein

The effect of the toxin on protoplasmic membrane protein was studied by the method of fluorescence,using 8-anilino-1-naphthalene-sulfonic acid(ANS) as probe and taking the mesophyll protoplasts from maize leaves as the research system.After the liquid of protoplasts labeled ANS was injected with different toxin conc.,dosage-effect relationship existed because of the ANS fluorescence intensity increasing obviously,which indicated the toxin had some kinds of effects on receptor of ANS.Two types of reagents were employed to prove the proteic characteristic of ANS receptor,the result of which demonstrated the proteic characteristic of the toxin.It was concluded that the first action site of the specific toxin might be on the outside of protoplasmic membrane,moreover,the toxin receptor has the proteic characteristic and the combination between toxin and its receptor has saturation activity.