分光光度法测定硝普钠注射液中硝普钠的含量

在碱性条件下， 在含有苯酚和盐酸羟胺的溶液中加入硝普钠， 有蓝色物质生成， 该物质在波长690 nm处有最大吸收. 由此建立了测定硝普钠注射液中硝普钠含量的新的分光 光度分析法. 该方法线性范围1.0×10^-5～8.0×10^-5 mol/L， 标 准溶液测定的相对标准偏差为0.57％（n=6）， 实际样品测定的回收率＞95%， 与标准方法 对照分析的相对误差为-1.2%～0.91% ， 40倍的Cl^-， SO^2-₄ ， HCO^－_３， Mg²⁺和Ca²⁺不干扰 测定.

Spectrophotometric Determination of Sodium Nitroprusside in Sodium Nitroprusside Injection Solution

In alkaline medium,　a reaction forming a blue product takes place among sodium nitroprusside, phenol and hydroxylamine hydrochloride. The blue produ ct exhibits the maximum absorbance at a wavelength of 690　nm and can be measured by spectrophotometry. So a spectrophotometric method is developed for the dete rmination of sodium nitroprusside in the injection solution of sodium nitroprusside. The linear range of the method is 1.0×10^-5～8.0×10^-5 mol/L,　RSD%=0.57％(n=6). The standard addition recoveries of the real samples ’ analyses are more than 95%. The relative errors between the proposed and the standard methods are -1.2%～0.91%. Cl^-, SO^2-₄, 　HCO^-_{3,　Mg2+ and Ca2+　 don’t show obvious disturbance when their concentrations are respectively 40 times higher than that of sodium nitroprusside. The method is precise and the operation is easy and convenient.}