Juvenile hormone catabolism inManduca sexta: Homologue selectivity of catabolism and identification of a diol-phosphate conjugate as a major end product |
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| Authors: | P. P. Halarnkar G. P. Jackson K. M. Straub D. A. Schooley |
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| Affiliation: | (1) Department of Biochemistry, University of Nevada, 89557 Reno, Nevada, USA;(2) Syntex Research, 94304 Palo Alto, California, USA |
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| Abstract: | We studied time-dependent metabolism of (10R)-[3H] juvenile hormone (JH) III and (10R, 11S)-[3H]JH I injected intoManduca sexta larvae; the hormones are metabolized to polar metabolites, expecially the JH acid-diol, and an unknown. Products were analyzed using a reversed-phase liquid chromatography assay. (10R)-JH III is metabolized much more rapidly than (10R, 11S)-[3H]JH I, whether injected seperately or as a mixture of hormones. The unknown metabolites of JH I and JH III were identified as phosphate conjugates of JH I and JH III diol by tandem mass spectral analysis of isolated samples. The phosphate conjugate of JH I diol is the principle end product of JH I metabolism. |
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| Keywords: | Juvenile hormone esterase juvenile hormone epoxide hydrolase juvenile hormone binding protein reversed-phase liquid chromatography assay |
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