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磁性聚乙二醇微球固定化辅酶维生素B6的研究
引用本文:杜崇旭,索朗措姆,范圣第. 磁性聚乙二醇微球固定化辅酶维生素B6的研究[J]. 大连民族学院学报, 2003, 5(1): 41-43,46
作者姓名:杜崇旭  索朗措姆  范圣第
作者单位:大连民族学院,生物工程系,辽宁,大连开发区,116600
摘    要:以制备的磁性聚乙二醇胶体粒子为载体,戊二醛为交联剂,固定化辅酶维生素B6,对固定化的条件进行了探索,确定了固定化的最佳条件:室温下,0.03g磁性聚乙二醇微球与5mL(8%)戊二醛交联,固定0.01g辅助酶维生素B6;振荡时间为12小时。

关 键 词:磁性聚乙二醇微球 固定化酶 维生素B6 戊二醛 生产工艺 固定化条件
文章编号:1009-315X(2003)01-0041-03

On the Immobilization of Magnetic Polyethylene Glycol with Vitamin B6
DU Chong-Xu,SUO LANG Cuo-Mu,FAN Sheng-Di. On the Immobilization of Magnetic Polyethylene Glycol with Vitamin B6[J]. Journal of Dalian Nationalities University, 2003, 5(1): 41-43,46
Authors:DU Chong-Xu  SUO LANG Cuo-Mu  FAN Sheng-Di
Abstract:Vitamin B6 is immobilized on magnetic polyethylene particle with glutaraldehyde by cross-linking reaction. The condition for immobilization and the characterization of the immobilized enzyme are studied. The optimal condition for immobilization is as follows:under room temperature, 0.03g magnetic polyethylene glycol microsphere is treated with 5ml(8%)glutaraldehyde, then 0.01g vitamin B6. The reaction time is 12h.
Keywords:magnetic polyethylene glycol microsphere  polyethylene glycol  immobilized enzyme  vitamin B6
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