| 磁性聚乙二醇微球固定化辅酶维生素B6的研究 |
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| 引用本文： | 杜崇旭,索朗措姆,范圣第.磁性聚乙二醇微球固定化辅酶维生素B6的研究[J].大连民族学院学报,2003,5(1):41-43,46. |
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| 作者姓名： | 杜崇旭 索朗措姆 范圣第 |
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| 作者单位： | 大连民族学院,生物工程系,辽宁,大连开发区,116600 |
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| 摘 要： | 以制备的磁性聚乙二醇胶体粒子为载体，戊二醛为交联剂，固定化辅酶维生素B6，对固定化的条件进行了探索，确定了固定化的最佳条件：室温下，0.03g磁性聚乙二醇微球与5mL(8%)戊二醛交联，固定0.01g辅助酶维生素B6；振荡时间为12小时。
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| 关 键 词： | 磁性聚乙二醇微球 固定化酶 维生素B6 戊二醛 生产工艺 固定化条件 |
| 文章编号： | 1009-315X(2003)01-0041-03 |
On the Immobilization of Magnetic Polyethylene Glycol with Vitamin B6 |
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| DU Chong-Xu,SUO LANG Cuo-Mu,FAN Sheng-Di.On the Immobilization of Magnetic Polyethylene Glycol with Vitamin B6[J].Journal of Dalian Nationalities University,2003,5(1):41-43,46. |
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| Authors: | DU Chong-Xu SUO LANG Cuo-Mu FAN Sheng-Di |
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| Abstract: | Vitamin B6 is immobilized on magnetic polyethylene particle with glutaraldehyde by cross-linking reaction. The condition for immobilization and the characterization of the immobilized enzyme are studied. The optimal condition for immobilization is as follows:under room temperature, 0.03g magnetic polyethylene glycol microsphere is treated with 5ml(8%)glutaraldehyde, then 0.01g vitamin B6. The reaction time is 12h. |
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| Keywords: | magnetic polyethylene glycol microsphere polyethylene glycol immobilized enzyme vitamin B6 |
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