重组骨形成蛋白—2与珊瑚人工骨复合物应用于拔牙窝修复的动物实验研究

目的：探讨重组骨形成蛋白－2（recombinant human bone morphogenetic protein rhBMP-2)/珊瑚人工骨复合物（复合骨）与珊瑚人工骨（珊瑚骨）在拔牙窝修复中的作用，方法：12只成年狗作为实验动物，拔除两侧上颌 第2及第3切牙，并去除牙槽窝之间的牙槽间 ，一侧随即植入复合骨，对侧植入珊瑚内作为对照，于并植骨后4，8，。12周取材，采用组织学观察及计算机图像分析方法，观察比较两种植入材料在拔牙窝内的骨修复能力及修复效果，结果：复合骨具有较强的骨修复作用，植入牙槽窝后，材料被逐渐降解吸收，新骨不断形成，12周后，植入材料完全被成熟的骨组织取代，图像分析结果显示复合骨组新生骨形成的比值明显高于珊瑚骨组，两组比较均有显著性差异（P＜0．05），结论：复合骨在拔牙窝中的骨修复能力和修复效果明显优于珊瑚骨。

The effects of coral artificial bone composite of recombinant human bone morphogenetic protein-2 on reconstruction of extraction sockets:an experimetal study on dogs

Aim:To evaluate the bone repairing ability of coral artificial bone composite of rhBMP-2 (rhBMP-2/CAB) and coral artificial bone(CAB) implanted into immediate extraction sockets. Methods: 12 adult dogs served as the experimental animals. Immediately after extraction of the upper second and third incisors, the alveolar septum between extraction sockets was resected bilaterally. RhBMP-2/CAB and CAB were implanted respectively into each extraction site. The animals were sacrificed at the 4 th, 8th and 12th weeks respectively after implantation. The bone repairing ability of the two grafts was analyzed with histologic and image analysis system. Results: RhBMP-2/CAB has a good effect on the repairing ability of extraction sockets. The implants were absorbed gradually after they were implanted into extraction sockets. In the meantime, the new bone was formed in extraction sockets. The implants were replaced completely by bone at 12 weeks. The ratio of new bone formation of rhBMP-2/CAB was significantly higher than that of CAB at different period( P <0.05). Conclusion: The repairing ability and effect of rhBMP-2/CAB in extraction sockets are obviously better than those of CAB