| Effect of HS2 and HS3 elements on erythroid-specific expression in transgenic mice |
| |
| 作者姓名： | JIA Chunping YAN Jingbin XIAO Yanping FANG Yudan HUANG Shuzheng ZENG Yitao |
| |
| 作者单位： | Institute of Medical Genetics, Shanghai Jiaotong University, Shanghai 200040, China,Institute of Medical Genetics, Shanghai Jiaotong University, Shanghai 200040, China,Institute of Medical Genetics, Shanghai Jiaotong University, Shanghai 200040, China,Institute of Medical Genetics, Shanghai Jiaotong University, Shanghai 200040, China,Institute of Medical Genetics, Shanghai Jiaotong University, Shanghai 200040, China,Institute of Medical Genetics, Shanghai Jiaotong University, Shanghai 200040, China |
| |
| 基金项目： | Supported by National 863 Hi-Tech Program of China (Grant No. 2002AA206211) and the National Natural Science Foundation of China (Grant No. 39893320) |
| |
| 摘 要： | The expression plasmids CMV/GFP, HS2ALL, HS3ALL and HS23ALL were selected to investigate the effect of HS2 and HS3 element on erythroid-specific expression in transgenic mice. These plasmids were digested with restriction enzymes and purified. And five DNA fragments, CMV/GFP, HS2/GFP, CMV/HS2/GFP, HS23/GFP and HS3/GFP were obtained. After purification, the above DNA fragments were microinjected into the pre-nuclei of the mice fertilized eggs and transgenic mice were generated, with an integration rate of 10.89%. The green fluorescence protein(GFP) expression in many transgenic mouse tissues was determined by FACS analysis. The results showed that the HS2 and 1.7 kb of β-globin gene promoter were sufficient for the erythroid-specific expression of β-globin gene. The GFP expression of different recombinant constructs was also analyzed in blood of all the transgenic mice with FACS. The results indicated that HS2 and HS3 had the same enhancement activity on the regulation of β-globin gene expression. Moreover, these two elements showed a significant synergistic effect on gene expression at the transgenic mouse level, although the GFP expression varied largely among different transgenic mouse litters.
|
| 关 键 词： | β-globin gene locus control region GFP transgenic mouse |
Effect of HS2 and HS3 elements on erythroid-specific expression in transgenic mice |
| |
| JIA Chunping,YAN Jingbin,XIAO Yanping,FANG Yudan,HUANG Shuzheng,ZENG Yitao.Effect of HS2 and HS3 elements on erythroid-specific expression in transgenic mice[J].Progress in Natural Science,2003,13(9):672-677. |
| |
| Authors: | JIA Chunping Yan Jingbin XIAO Yanping FANG Yudan HUANG Shuzheng ZENG Yitao |
| |
| Institution: | Institute of Medical Genetics, Shanghai Jiaotong University, Shanghai 200040, China |
| |
| Abstract: | The expression plasmids CMV/GFP, HS2ALL, HS3ALL and HS23ALL were selected to investigate the effect of HS2 and HS3 element on erythroid-specific expression in transgenic mice. These plasmids were digested with restriction enzymes and purified. And five DNA fragments, CMV/GFP, HS2/GFP, CMV/HS2/GFP, HS23/GFP and HS3/GFP were obtained. After purification, the above DNA fragments were microinjected into the pre-nuclei of the mice fertilized eggs and transgenic mice were generated, with an integration rate of 10.89%. The green fluorescence protein(GFP) expression in many transgenic mouse tissues was determined by FACS analysis. The results showed that the HS2 and 1.7 kb of β-globin gene promoter were sufficient for the erythroid-specific expression of β-globin gene. The GFP expression of different recombinant constructs was also analyzed in blood of all the transgenic mice with FACS. The results indicated that HS2 and HS3 had the same enhancement activity on the regulation of β-globin gene expression. Moreover, these two elements showed a significant synergistic effect on gene expression at the transgenic mouse level, although the GFP expression varied largely among different transgenic mouse litters. |
| |
| Keywords: | locus control region GFP transgenic mouse |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《自然科学进展(英文版)》浏览原始摘要信息 |
| 点击此处可从《自然科学进展(英文版)》下载免费的PDF全文 |
|
