| 果胶酶高产菌株EIM-6的筛选鉴定及其液体发酵产酶条件优化 |
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| 引用本文： | 杨欣伟,林伟铃,田宝玉,柯崇榕,黄薇,黄建忠. 果胶酶高产菌株EIM-6的筛选鉴定及其液体发酵产酶条件优化[J]. 福建师范大学学报(自然科学版), 2009, 25(2) |
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| 作者姓名： | 杨欣伟 林伟铃 田宝玉 柯崇榕 黄薇 黄建忠 |
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| 作者单位： | 福建师范大学工业微生物教育部工程研究中心,福建省现代发酵技术工程研究中心,福建,福州,350108 |
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| 基金项目： | 福建省科技厅重大项目 |
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| 摘 要： | 通过筛选得到一株适合液体深层发酵培养的高产果胶酶菌株EIM-6,基于形态学与ITS序列分子系统进化分析,鉴定为Aspergillus niger.运用单因子实验确定EIM-6产果胶酶最适碳源和氮源.在此基础上,通过Plackett-Burrman设计对影响其产酶的相关因素进行评估并筛选出具有显著效应的3个因素.然后通过最陡爬坡实验在上升最高点处由中心组合实验和响应面分析确定其最适产酶条件.实验优化的最优产酶条件为:w(橘皮粉)4.09%,w(麸皮)3.16%,w((NH4)2SO4)0.35%,w(K2HPO4)0.3%,w(CaCO3)0.24%,初始pH 6,接种量107/mL,转速250 r/min,28 ℃培养80 h酶活达30 231 U/mL,与初始相比,酶活提高2.07倍.
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| 关 键 词： | 黑曲霉 果胶酶 分子鉴定 Plackett-Burrman设计 响应面分析法 |
Identification of Aspergillus niger EIM-6 with High-yield of Pectinase and Optimization of Its Submerged Fermentation Conditions by Response Surface Methodology |
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| YANG Xin-wei,LIN Wei-lin,TIAN Bao-yu,KE Chong-rong,HUANG Wei,HUANG Jian-zhong. Identification of Aspergillus niger EIM-6 with High-yield of Pectinase and Optimization of Its Submerged Fermentation Conditions by Response Surface Methodology[J]. Journal of Fujian Teachers University(Natural Science), 2009, 25(2) |
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| Authors: | YANG Xin-wei LIN Wei-lin TIAN Bao-yu KE Chong-rong HUANG Wei HUANG Jian-zhong |
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| Affiliation: | Engineering Research Center of Industrial Microbiology;Ministry of Education;Fujian Normal University;Engineering Research Center of Fujian Modern Fermentation Technology;Fuzhou 350108;China |
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| Abstract: | A highly pectinase-producing strain EIM-6 was isolated and identified as Aspergillus niger based on its phylogenetic analysis of ITS sequence.Through single-factor experiments,the optimum carbon and nitrogen sources for EIM-6 to produce pectinase in shake-flask cultures were obtained,subsequently some process factors under submerged fermentation conditions were evaluated and three factors demonstrated prominent effect on pectinase production,respectively by Plackett-Burrman design.The results showed that th... |
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| Keywords: | Aspergillus niger pectinase molecular phylogenetic analysis Plackett-Burrman design response surface methodology |
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