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| 几个猪卵母细胞体外培养和受精的影响 | |
| 引用本文： | 徐飞良,肖兵南,胡兴旺,李剑波,燕海峰,张健.几个猪卵母细胞体外培养和受精的影响[J].湖南师范大学自然科学学报,2007,30(1):88-92. |
| 作者姓名： | 徐飞良 肖兵南 胡兴旺 李剑波 燕海峰 张健 |
| 作者单位： | 1. 湖南省畜牧兽医研究所,中国,长沙,410131;湖南农业大学动物科技学院,中国,长沙,410128 2. 湖南省畜牧兽医研究所,中国,长沙,410131 3. 湖南师范大学生命科学学院,中国,长沙,410081 |
| 基金项目： | 湖南省教育厅、财政厅、科技厅、发改委专项基金资助项目(03G2Y1003) |
| 摘 要： | 比较了卵母细胞采集方法(剖解法,抽吸法,机械破碎法)、卵母细胞体外成熟培养方法以及猪卵泡液(PFF)质量浓度对猪卵母细胞培养和体外受精的影响.结果表明:采取剖解法采集猪卵母细胞、在猪卵母细胞成熟培养液中添加10%的PFF为最佳方法.微滴法与平皿法两种培养方法对猪卵母细胞培养和体外受精的影响无显著差异. |
| 关 键 词： | 猪 体外受精 卵母细胞体外培养 |
| 文章编号： | 1000-2537（2006）01-0088-05 |
| 收稿时间： | 2006-08-02 |
| 修稿时间： | 08 2 2006 12:00AM |
Affecting in Vitro Culture and Fertilization of Porcine Oocytes |
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| XU Fei-liang,XIAO Bin-nan,HU Xing-wang,LI Jian-bo,YAY Hai-feng,ZHANY Jian.Affecting in Vitro Culture and Fertilization of Porcine Oocytes[J].Journal of Natural Science of Hunan Normal University,2007,30(1):88-92. | |
| Authors: | XU Fei-liang XIAO Bin-nan HU Xing-wang LI Jian-bo YAY Hai-feng ZHANY Jian |
| Institution: | 1. Hunan Institute of Animal and Veterinary Science, Changsha 410131 ,China; 2. College of Atfimal Science, Hunan Agriculture University, Changsha 410128, China ; 3. College of Life Science, Hunan Normal University, Changsha 410081, China |
| Abstract: | The effects of oocyte-collecting method(aspiration,dissection,mechanical break-up),in vitro oocyte maturation culture method(plate culture,droplet culture),and porcine follicular fluid(PFF) concentration in culture medium to porcine oocyte culture and in vitro fertilization was compared.The results shown that using dissection method to collect oocytes and adding 10% PFF in culture medium are optimal condition for porcine oocyte in vitro maturation and fertilization,while there are no significant different effects between plate culture and droplet culture. |
| Keywords: | porcine in vitro fertilization in vitro oocyte culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |