| 光谱法研究钙黄绿素与鲱鱼精DNA的相互作用 |
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| 引用本文： | 黎泓波,张晓宇,王兴明,游斌,王丹名,石荣铭,丁立生,董发勤.光谱法研究钙黄绿素与鲱鱼精DNA的相互作用[J].湖南师范大学自然科学学报,2007,30(1):47-51. |
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| 作者姓名： | 黎泓波 张晓宇 王兴明 游斌 王丹名 石荣铭 丁立生 董发勤 |
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| 作者单位： | 1. 西南科技大学材料科学与工程学院,中国,绵阳,621010
2. 中国科学院成都生物研究所,中国,成都,610041 |
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| 基金项目： | 国家自然科学基金资助项目(No.30572254) |
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| 摘 要： | 本实验在接近生理条件下,以吖啶橙(AO)作为光谱探针,采用UV-Vis光谱、荧光光谱、热变性等方法研究了钙黄绿素(CA)与鲱鱼精DNA的作用机制.确定了钙黄绿素(CA)与鲱鱼精DNA之间主要以嵌插方式作用,钙黄绿素(CA)能对鲱鱼精DNA的生物功能产生一定影响.
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| 关 键 词： | 钙黄绿素 吖啶橙 鲱鱼精DNA |
| 文章编号： | 1000-2537（2007）01-0047-05 |
| 收稿时间： | 2006-12-05 |
| 修稿时间： | 12 5 2006 12:00AM |
Study on the Interaction between Calcein and Herring Sperm DNA by Spectrometry |
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| LI Hong-bo,ZHANG Xiao-yu,WANG Xing-ming,YOU Bin,WANG Dan-ming,SHI Rong-ming,DING Li-sheng,DONG Fa-qin.Study on the Interaction between Calcein and Herring Sperm DNA by Spectrometry[J].Journal of Natural Science of Hunan Normal University,2007,30(1):47-51. |
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| Authors: | LI Hong-bo ZHANG Xiao-yu WANG Xing-ming YOU Bin WANG Dan-ming SHI Rong-ming DING Li-sheng DONG Fa-qin |
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| Institution: | 1. Department of Chemistry, Southwest University of Science and Technology, Mianyang 621010, China; 2 .Chengdu Institute of Biology, Chinese Academy of Sciences, Chnngdu 610041 ,China |
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| Abstract: | The mechianism of interaction between calcein(CA) and herring sperm DNA has been studied by using acridine orange(AO) as a spectral probe with the ways of UV-Vis spectra,fluorescence spectra and melting curve in buffer solution of Tris-HCl(pH=7.00).The experiments comfirmed that calcein with DNA mainly by intercalative and suggest that the biology function of DNA was changed. |
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| Keywords: | calcein Acridine orange herring sperm DNA |
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