| 紫外分光光度法检测桔皮中总黄酮含量的方法研究 |
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| 引用本文： | 张浩,郑妮.紫外分光光度法检测桔皮中总黄酮含量的方法研究[J].合肥学院学报(自然科学版),2013(4):55-57. |
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| 作者姓名： | 张浩 郑妮 |
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| 作者单位： | [1]合肥学院,生物与环境工程系,合肥230601 [2]新华学院,动漫学院,合肥230088 |
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| 摘 要： | 为了研究紫外分光光度法测定桔皮中总黄酮含量的可行性.通过精密度、重复性、稳定性及加标回收率等方面的考察确定此方法是否可行.实验结果证明相关性良好,R2=0.999 9,线性范围在0.01~0.05 g/L,加标回收率为99.11%~100.78%,实验结果良好,稳定性RSD=1.34%.因此,紫外分光光度法快速、准确,可以作为桔皮中总黄酮含量的测定方法.
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| 关 键 词： | 桔皮 总黄酮 紫外分光光度法 |
Determination of Total Flavonoids Content in Citrus Peels by Ultraviolet Spectrophotometry |
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| ZHANG Hao,ZHENG Ni.Determination of Total Flavonoids Content in Citrus Peels by Ultraviolet Spectrophotometry[J].Journal of Hefei University :Natural Sciences,2013(4):55-57. |
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| Authors: | ZHANG Hao ZHENG Ni |
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| Institution: | 1. Department of Biological and Environmental Engineering,Hefei University, Hefei 230601 ; 2. School of Animation, Xinhua University, Hefei 230088, China) |
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| Abstract: | In order to study the measurement of total flavonoids content in citrus peels by ultraviolet spectrophotometry and make methodological observation. Based on the contents of linear range,precision, repeatability, stability and the average recovery, the method was studied whether it was feasible. This method had good con＇elation（ R 2 =0. 999 9） and the linear range was 0. 01 -0. 05 g/L. The result of recovery experiment was great and the stability（ RSD = 1. 34% ） was favorable. So using UV spectrophotometry to detect the content of total flavonoids content in citrus peels is a kind of simple and accurate method. |
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| Keywords: | orange peel total flavonoids ultraviolet spectrophotometry |
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