| 模拟酶轴向配体激活过氧化氢酶的研究──提高天然酶活性的新方法 |
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| 引用本文： | 刘建忠,杨惠英,赵继伦,翁丽萍,计亮年.模拟酶轴向配体激活过氧化氢酶的研究──提高天然酶活性的新方法[J].中山大学学报(自然科学版),1998(2). |
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| 作者姓名： | 刘建忠 杨惠英 赵继伦 翁丽萍 计亮年 |
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| 作者单位： | 中山大学生物工程研究中心，华南理工大学生物工程系 |
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| 摘 要： | 曾报道了以铁卟啉作为细胞色素Ｐ４５０模拟酶的活性中心，轴向配体例如巯基苯甲酸、半胱氨酸作为激活剂，提高模拟酶体系催化活性的研究．而过氧化氢酶的活性中心也为铁卟啉．进一步报道模拟酶的轴向配体提高过氧化氢酶的活性，并提出了一种提高天然酶活性的新方法．硫代硫酸钠、维生素Ｃ、巯基乙酸和Ｌ－半胱氨酸可作为过氧化氢酶的轴向配体，而激活过氧化氢酶．
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| 关 键 词： | 过氧化氢酶，轴向配体，酶激活 |
A Study on Axial Ligands of Model Enzyme to Activize Catalase A New Method of Enhancement Natural Enzyme Activity |
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| Liu JianzhongBiotechnology Research Center,Zhongshan University,Guangzhou ,China Yang Huiying Zhao Jilun Weng Liping Ji Liangnian.A Study on Axial Ligands of Model Enzyme to Activize Catalase A New Method of Enhancement Natural Enzyme Activity[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,1998(2). |
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| Authors: | Liu JianzhongBiotechnology Research Center Zhongshan University Guangzhou China Yang Huiying Zhao Jilun Weng Liping Ji Liangnian |
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| Institution: | Liu JianzhongBiotechnology Research Center,Zhongshan University,Guangzhou 510275,China Yang Huiying Zhao Jilun Weng Liping Ji Liangnian |
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| Abstract: | Ironic porphyrin as an active center of model enzyme of cytochrome P450 and axial ligands which were thiosalicylic acid,L cystein, etc. and can enhance catalytic activity of model system was reported. The active center of catalase is also ironic porphyrin.This paper reports further that axial ligands of model enzyme can enhance similarly activity of catalase and put forward a new method of enhancement natural enzyme activity.Sodium thiosulfate,vitamin C,thioglycolic acid,and L cystein can activize catalase as an axial ligand of its iron porphyrin,respectively. |
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| Keywords: | catalase axial ligands enzyme activation |
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