A nonenzymic oxygen uptake and its implications in the assay of 4-hydroxyphenylpyruvate dioxygenase by an oxygen electrode |
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| Authors: | P. J. Evans |
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| Affiliation: | (1) Biochemistry Laboratory, Department of Zoology, University of Oxford, South Parks Road, OX1 3PS Oxford, UK;(2) Department of Biochemistry, University of Nottingham Medical School, Queen's Medical Centre, NG7 2UH Nottingham, UK |
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| Abstract: | Summary The oxygen electrode provides a rapid, convenient assay for 4-hydroxyphenylpyruvate dioxygenase. However, due to the occurrence of a nonenzymic oxygen consumption when reducing agents were mixed witha,a -dipyridyl, its use is restricted to species which do not require reducing agents or necessitates the addition of catalase to the reaction mixture. |
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