| 湘西宏成制药公司不同基地泡桐的分子鉴定 |
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| 引用本文： | 李科,吾鲁木杆·那孜尔别克,张代贵,姚福成,恩特马克·布拉提白.湘西宏成制药公司不同基地泡桐的分子鉴定[J].吉首大学学报(自然科学版),2011,32(4):83-87. |
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| 作者姓名： | 李科 吾鲁木杆·那孜尔别克 张代贵 姚福成 恩特马克·布拉提白 |
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| 作者单位： | （1.吉首大学生物资源与环境科学学院，湖南 吉首 416000；2.湘西宏成制药有限公司，湖南 吉首 416000） |
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| 基金项目： | 湖南省研究生科技创新项目(CX2009B198); 湘西宏成制药公司科技开发项目(XHZYP200921) |
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| 摘 要： | 根据核rRNA基因ITS序列、叶绿体trnL-trnF和psbA-trnH序列对湘西宏成制药公司不同基地泡桐进行分子鉴定.用PCR从泡桐基因组DNA中分别扩增出大小约为500bp的ITS序列、540bp的psbA-trnH序列和900bp的trnL-trnF序列.相似性分析结果表明,毛泡桐ITS序列与1号和2号基地泡桐...
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| 关 键 词： | 泡桐 ITS序列 psbA-trnH序列 trnL-trnF序列 分子鉴定 |
Molecular Identification of Paulownia Plants from Xiangxi Hongcheng Pharmaceutical Companies |
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| LI ke,NAZIERBIEKE Wulumuhan,ZHANG Dai-gui,YAO Fu-chun,BORRATHYBAY Entomack.Molecular Identification of Paulownia Plants from Xiangxi Hongcheng Pharmaceutical Companies[J].Journal of Jishou University(Natural Science Edition),2011,32(4):83-87. |
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| Authors: | LI ke NAZIERBIEKE Wulumuhan ZHANG Dai-gui YAO Fu-chun BORRATHYBAY Entomack |
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| Institution: | (1.College of Biology and Environmental Sciences,Jishou University,Jishou 416000,Hunan China;2.Xiangxi Hongcheng Pharmaceutical Co.,LTD,Jishou 416000,Hunan China) |
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| Abstract: | The genomic DNA was isolated from leaves of Paulownia plants through the CTAB method without liquid nitrogen.The internal transcribed spacer(ITS) of rRNA and two intergenic spacers of psbA-trnH and trnF-trnF were amplified from the genomic DNA by PCR and then sequenced.Sequence analysis of the three DNA markers were conducted with software Clustal X7.0,and their phylogenetic trees were constructed with software MEGA3.1.The results showed three fragments of ITS,psbA-trnH and trnL-trnF was 600 bp,540 bp and 9... |
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| Keywords: | Paulownia ITS psbA-trnH trnL-trnF molecular identification |
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