| 反相高效液相色谱法(RP-HPLC)测定丙酮酸发酵液中丙酮酸 |
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| 引用本文： | 高年发,吴迪,张健. 反相高效液相色谱法(RP-HPLC)测定丙酮酸发酵液中丙酮酸[J]. 天津科技大学学报, 2006, 21(1): 21-24 |
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| 作者姓名： | 高年发 吴迪 张健 |
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| 作者单位： | 天津市工业微生物重点实验室,天津科技大学生物工程学院,天津,300222 |
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| 摘 要： | 提出一种利用反向高效液相色谱法(RP-HPLC)测定丙酮酸发酵液中丙酮酸的方法。应用反相C18色谱柱 ODS-2 HYPERSIL 250 mm×4．6 mm 5μm,以0．05 mol·L-1NH4H2PO4(用H3PO4调pH2．6)为流动相,发酵液经稀硫酸预处理后直接进样分离定量,每一样品的分析过程不超过5 min,可及时了解丙酮酸发酵过程中丙酮酸的变化,适于发酵液中丙酮酸的检测。
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| 关 键 词： | 反向高效液相色谱法 丙酮酸发酵液 丙酮酸 |
| 文章编号： | 1672-6510(2006)01-0021-04 |
| 收稿时间： | 2005-05-13 |
| 修稿时间： | 2005-05-13 |
Determination of Pyruvate in Pyruvate Fermentation Broth by Reversed phase High-performance Liquid Chromatography (RP-HPLC) |
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| GAO Nian-fa,WU Di,ZHANG Jian. Determination of Pyruvate in Pyruvate Fermentation Broth by Reversed phase High-performance Liquid Chromatography (RP-HPLC)[J]. Journal of Tianjin University of Science & Technology, 2006, 21(1): 21-24 |
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| Authors: | GAO Nian-fa WU Di ZHANG Jian |
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| Abstract: | A RP-HPLC method for the determination of pyruvate in pyruvate fermentation broth was developed. ODS-2 HY-PERSIL C18 column was used and mobile phase was 0.05 mol/L NH4H2PO4 (adjusted to pH 2.6 with H3PO4). The solution of samples pre-treated with the solution of sulfuric acid would be determined, The analysis process would be finished in five minutes. The method was proved a rapid effective way to determine the pyruvate in pyruvate fermentation broth. |
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| Keywords: | reversed-phase high performance liquid chromatograph(RP-HPLC) pyruvate fermentation broth pyruvate |
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