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硫氧还蛋白f介导的烟草Rubisco活化酶的氧化还原调控
引用本文:孙逸,陈娟,马为民,陈根云,米华玲. 硫氧还蛋白f介导的烟草Rubisco活化酶的氧化还原调控[J]. 上海师范大学学报(自然科学版), 2014, 43(2): 188-195
作者姓名:孙逸  陈娟  马为民  陈根云  米华玲
作者单位:1. 上海师范大学生命与环境科学学院,上海,200234
2. 中国科学院上海生命科学研究院植物生理生态研究所,上海,200032
基金项目:国家重点基础研究发展计划
摘    要:利用菠菜和烟草的硫氧还蛋白f(Trx-f)在不同氧化还原状态下测定了菠菜、拟南芥、烟草和番茄Rubisco活化酶(RCA)活化其Rubisco的活力,实验结果显示菠菜Trx-f只能介导调控菠菜和拟南芥RCA的氧化还原状态,从而改变其活力,而烟草Trx-f介导也只能改变烟草和番茄RCA的氧化还原状态.上述结果表明烟草等物种的单亚型RCA也能受Trx-f介导的氧化还原调控,而且Trx-f介导的RCA氧化还原状态的改变具有种属专一性.

关 键 词:硫氧还蛋白f烟草  Rubsico活化酶  光合作用  氧化还原调控
收稿时间:2013-12-05

Redox regulation of Tobacco Rubisco activase mediated by thioredoxin-f
SUN Yi,CHEN Juan,MA Weimin,CHEN Genyun and MI Hualing. Redox regulation of Tobacco Rubisco activase mediated by thioredoxin-f[J]. Journal of Shanghai Normal University(Natural Sciences), 2014, 43(2): 188-195
Authors:SUN Yi  CHEN Juan  MA Weimin  CHEN Genyun  MI Hualing
Affiliation:SUN Yi;CHEN Juan;MA Weimin;CHEN Genyun;MI Hualing;College of Life and Environmental Sciences,Shanghai Normal University;Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Science;
Abstract:We measured the activation activities of RCA from spinach,arabidopsis,tobacco and tomato under different redox states in the presence of Trx-f from spinach and tobacco,respectively. The results showed that the activity of RCA from spinach and Arabidopsis was regulated by different redox states in the presence of Trx-f from spinach,and the activity of RCA from tobacco and tomato was regulated by different redox states in the presence of Trx-f from tobacco. It demonstrated that that both the activity of RCA consisted of two isoforms and only one isoform(like tobacco) can be regulated by changes in the redox states,which mediated by the Trx-f. Furthermore,we also suggested changes in the redox state of RCA mediated by Trx-f are species dependent.
Keywords:thioredoxin-f tobacco  Rubsico activase  photosynthesis  redox regulation
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