| ELISA和PCR法在研究乙肝病毒传播途径中的应用 |
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| 引用本文： | 张荣波,王克霞,王健,张宁. ELISA和PCR法在研究乙肝病毒传播途径中的应用[J]. 安徽理工大学学报(自然科学版), 1998, 0(2) |
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| 作者姓名： | 张荣波 王克霞 王健 张宁 |
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| 作者单位： | 淮南工业学院医学分院(张荣波，王克霞，张宁)，淮南铁路医院(王健) |
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| 摘 要： | 应用HBV表面抗原酶标试剂盒和诊断HBVDNA的PCR试剂盒分别检测可能污染有HBV的不同来源标本30份，结果ELISA法检出HBsAg阳性5例，检出率16．7％；PCR法检出HBVDNA阳性12例，检出率40％，明显高于ELISA法，提示应用PCR技术探讨乙肝病毒传播途径似乎效果更好。
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| 关 键 词： | 酶联免疫吸附试验 聚合酶链反应 乙型肝炎病毒 |
Application of ELISA and PCR in study of the mode of transmission of hepatitis B virus |
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| Zhang Rongbo, Wang Kexia, Wang Jian, Zhang Ning. Application of ELISA and PCR in study of the mode of transmission of hepatitis B virus[J]. Journal of Anhui University of Science and Technology:Natural Science, 1998, 0(2) |
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| Authors: | Zhang Rongbo Wang Kexia Wang Jian Zhang Ning |
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| Affiliation: | Zhang Rongbo; Wang Kexia; Wang Jian; Zhang Ning (Medical College) (Huainan Railway Hospital) (Medical College) |
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| Abstract: | Polymerase chain reaction(PCR) technique was used to study hepatitis B virus (HBV) DNA, and enzyme-linked immune sorbent assay (ELISA) was adopted to detect HBsAg in the 30 different samples in which 12 cases were HBVDNA positive by ELISA,with rate of 40% and 5 were HBsAg positly by ELISA,with rate of 16. 7%. This result indicates that the PCR superior to the ELISA in detection of hapertitis B virus. |
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| Keywords: | enzyme-linked immune sorbent assay polymerase chain reaction hepatitis |
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