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ELISA和PCR法在研究乙肝病毒传播途径中的应用
引用本文:张荣波,王克霞,王健,张宁. ELISA和PCR法在研究乙肝病毒传播途径中的应用[J]. 安徽理工大学学报(自然科学版), 1998, 0(2)
作者姓名:张荣波  王克霞  王健  张宁
作者单位:淮南工业学院医学分院(张荣波,王克霞,张宁),淮南铁路医院(王健)
摘    要:应用HBV表面抗原酶标试剂盒和诊断HBVDNA的PCR试剂盒分别检测可能污染有HBV的不同来源标本30份,结果ELISA法检出HBsAg阳性5例,检出率16.7%;PCR法检出HBVDNA阳性12例,检出率40%,明显高于ELISA法,提示应用PCR技术探讨乙肝病毒传播途径似乎效果更好。

关 键 词:酶联免疫吸附试验  聚合酶链反应  乙型肝炎病毒

Application of ELISA and PCR in study of the mode of transmission of hepatitis B virus
Zhang Rongbo, Wang Kexia, Wang Jian, Zhang Ning. Application of ELISA and PCR in study of the mode of transmission of hepatitis B virus[J]. Journal of Anhui University of Science and Technology:Natural Science, 1998, 0(2)
Authors:Zhang Rongbo   Wang Kexia   Wang Jian   Zhang Ning
Affiliation:Zhang Rongbo; Wang Kexia; Wang Jian; Zhang Ning (Medical College) (Huainan Railway Hospital) (Medical College)
Abstract:Polymerase chain reaction(PCR) technique was used to study hepatitis B virus (HBV) DNA, and enzyme-linked immune sorbent assay (ELISA) was adopted to detect HBsAg in the 30 different samples in which 12 cases were HBVDNA positive by ELISA,with rate of 40% and 5 were HBsAg positly by ELISA,with rate of 16. 7%. This result indicates that the PCR superior to the ELISA in detection of hapertitis B virus.
Keywords:enzyme-linked immune sorbent assay  polymerase chain reaction  hepatitis
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