| Transmission electron microscopy and atomic force microscopy characterization of nickel deposition on bacterial cells |
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| 作者姓名： | WANG Jing HE ShiYing XU LiNa GU Ning |
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| 作者单位： | Jiangsu Laboratory for Biomaterials and Devices, National Laboratory of Molecular and Biomolecular Electronics, Department ofBiological Science and Medical Engineering, Southeast University, Nanjing 210096, China |
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| 基金项目： | Supported by the National Natural Science Foundation of China （Grant Nos. 60171005, 60371027, 6012110h 20573019 and 90406023） and 0pen Project Foundation of Laboratory of Solid State Microstructures of Nanjing University.The authors would like to thank Xu Aiqun for TEM measurement and valuable discussion. |
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| 摘 要： | Recently bacterial cells have become attractive biological templates for the fabrication of metal nano- structures or nanomaterials due to their inherent small size, various standard geometrical shapes and abundant source. In this paper, nickel-coated bacterial cells (gram-negative bacteria of Escherichia coli) were fabricated via electroless chemical plating. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) characterization results reveal evident morphological difference between bacterial cells before and after deposition with nickel. The bare cells with smooth surface presented transverse outspreading effect at mica surface. Great changes took place in surface roughness for those bacterial cells after metallization. A large number of nickel nanoparticles were observed to be equably distributed at bacterial surface after activation and subsequent metallization. Furthermore, ultra thin section analytic results validated the presence and uniformity of thin nickel coating at bacte- rial surface after metallization.
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| 关 键 词： | 原子粒显微镜 电子显微镜 金属化 细菌细胞 |
| 收稿时间： | 4 January 2007 |
| 修稿时间： | 2007-01-04 |
Transmission electron microscopy and atomic force microscopy characterization of nickel deposition on bacterial cells |
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| WANG Jing HE ShiYing XU LiNa GU Ning.Transmission electron microscopy and atomic force microscopy characterization of nickel deposition on bacterial cells[J].Chinese Science Bulletin,2007,52(21):2919-2924. |
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| Authors: | Wang Jing He ShiYing Xu LiNa Gu Ning |
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| Institution: | (1) Jiangsu Laboratory for Biomaterials and Devices, National Laboratory of Molecular and Biomolecular Electronics, Department of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China |
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| Abstract: | Recently bacterial cells have become attractive biological templates for the fabrication of metal nano- structures or nanomaterials due to their inherent small size, various standard geometrical shapes and abundant source. In this paper, nickel-coated bacterial cells (gram-negative bacteria of Escherichia coli) were fabricated via electroless chemical plating. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) characterization results reveal evident morphological difference between bacterial cells before and after deposition with nickel. The bare cells with smooth surface presented transverse outspreading effect at mica surface. Great changes took place in surface roughness for those bacterial cells after metallization. A large number of nickel nanoparticles were observed to be equably distributed at bacterial surface after activation and subsequent metallization. Furthermore, ultra thin section analytic results validated the presence and uniformity of thin nickel coating at bacte- rial surface after metallization. |
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| Keywords: | biotemplate atomic force microscopy (AFM) transmission electron microscopy (TEM) metallization bacterial cell |
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