| Transpositional behaviour of the Ds element in the Ac/Ds system in rice |
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| 作者姓名： | LIU Fang ZHANG XiangQian ZHANG ZeMin CHEN ZhaoGui ZHU HaiTao WANG Jiang ZHANG JingLiu ZHANG GuiQuan |
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| 作者单位： | [1]College of Agriculture, South China Agricultural University, Guangzhou 510642, China [2]National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China |
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| 基金项目： | Supported by National Basic Research Program of China （Grant No, G19990116） |
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| 摘 要： | Insertional mutagenesis based on maize Activator/Dissociator (Ac/Ds) transposons is becoming a ma- jor approach used to produce a saturated mutant collection in rice. In this research, Ds-T-DNA trans- formed homozygotes were crossed with Ac-T-DNA transformed homozygotes in order to establish an Ac/Ds transposon system in rice. The successive investigation of Ds transposition from F1 to F5 gen- erations indicated that the frequencies of germinal transposition increased over successive genera- tions and reached 54.2% in F3 generation. The Ds transposition pattern revealed that a Ds transposition induced an approximately 170-bp deletion of T-DNA sequence and another Ds transposition carried a 272-bp T-DNA sequence. Using thermal asymmetric interlaced PCR (TAIL-PCR), some flanking se- quences of the Ds element were amplified. Analyses of 17 Ds-flanking sequences showed that five Ds were inserted into gene regions. The Ds could transpose not only to the linked sites but also to the unlinked sites. The frequency of inter-chromosomal transposition of Ds was 33.3%.
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| 关 键 词： | 置换行为 稻米 激活剂 幼芽 |
| 收稿时间： | 28 January 2007 |
| 修稿时间： | 2007-01-28 |
Transpositional behaviour of the <Emphasis Type="Italic">Ds</Emphasis> element in the <Emphasis Type="Italic">Ac</Emphasis>/<Emphasis Type="Italic">Ds</Emphasis> system in rice |
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| LIU Fang ZHANG XiangQian ZHANG ZeMin CHEN ZhaoGui ZHU HaiTao WANG Jiang ZHANG JingLiu ZHANG GuiQuan.Transpositional behaviour of the Ds element in the Ac/Ds system in rice[J].Chinese Science Bulletin,2007,52(20):2789-2796. |
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| Authors: | Liu Fang Zhang XiangQian Zhang ZeMin Chen ZhaoGui Zhu HaiTao Wang Jiang Zhang JingLiu Zhang GuiQuan |
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| Institution: | (1) College of Agriculture, South China Agricultural University, Guangzhou, 510642, China;(2) National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai, 200032, China |
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| Abstract: | Insertional mutagenesis based on maize Activator/Dissociator (Ac/Ds) transposons is becoming a ma- jor approach used to produce a saturated mutant collection in rice. In this research, Ds-T-DNA trans- formed homozygotes were crossed with Ac-T-DNA transformed homozygotes in order to establish an Ac/Ds transposon system in rice. The successive investigation of Ds transposition from F1 to F5 gen- erations indicated that the frequencies of germinal transposition increased over successive genera- tions and reached 54.2% in F3 generation. The Ds transposition pattern revealed that a Ds transposition induced an approximately 170-bp deletion of T-DNA sequence and another Ds transposition carried a 272-bp T-DNA sequence. Using thermal asymmetric interlaced PCR (TAIL-PCR), some flanking se- quences of the Ds element were amplified. Analyses of 17 Ds-flanking sequences showed that five Ds were inserted into gene regions. The Ds could transpose not only to the linked sites but also to the unlinked sites. The frequency of inter-chromosomal transposition of Ds was 33.3%. |
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| Keywords: | Oryza sativa Ac Ds somatic transposition germinal transposition |
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