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Further studies on gel filtration of urinary substances
Authors:J A Kofoed  A C Barcelo  A A Tocci
Institution:(1) Catedra de Fisiologia, Facultad de Odontologia, Universidad de Buenos Aires, M. T. de Alvear 2142, Buenos Aires, (Argentina);(2) Present address: Consejo Nacional de Investigaciones Cientificas y Técnicas, República Argentina
Abstract:Summary Urine specimens from healthy children were concentrated and filtered through Sephadex G-100 and G-25. The eluate was recovered in fractions of 5 ml each. Total hexoses, galactose, total proteins and total uronic acids were determined on each fraction. Total hexoses and galactose were eluted in 6 distinct peaks. The uronic acid containing GAG were eluted in 4 small peaks on G-25 and 1 peak on G-100. This peak is eluted always with high amounts of galactose containing glycoproteins.This work was partially supported by research grants from the CONICET, República Argentina.
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