Development of DArT markers for a linkage map of flue-cured tobacco |
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Authors: | XiuPing Lu YiJie Gui BingGuang Xiao YongPing Li ZhiJun Tong Yun Liu XueFei Bai WeiRen Wu Ling Xia Eric Huttner Adrzej Kilian LongJiang Fan |
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Institution: | 1. Department of Agronomy, Zhejiang University, Hangzhou, 310058, China 2. Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Center at Yunnan, Yuxi, 653100, China 3. DArT P/L, PO Box 7141, Yarralumla, Canberra, ACT, 2600, Australia
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Abstract: | Tobacco (Nicotiana tabacum) is one of the most economically important nonfood crops, and flue-cured tobacco accounts for approximately 80% of world tobacco production. An extremely narrow genetic diversity in the tobacco pool has led to a low efficiency of PCR-based molecular markers (such as AFLP and SSR). Diversity Arrays Technology (DArT) is a high-throughput hybridisation-based marker system that has been developed in many plants including wheat, which, like tobacco, has a complex genome. In this study, we developed a tobacco DArT chip that included 7680 representative sequence tags based on typical tobacco accessions. The 1076 DArT markers of flue-cured tobacco were identified and most (82.1%) of their polymorphism information contents (PICs) were greater than 0.4. An integrated linkage map that included 851 markers (238 DArT and 613 SSR), which is the highest density map of flue-cured tobacco to date, was constructed. This chip-based DArT system provides an alternative in high-throughput marker genotyping for tobacco. |
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