| CTLA4Ig真核表达质粒及穿梭质粒的构建 |
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| 引用本文： | 张进安,杨章民.CTLA4Ig真核表达质粒及穿梭质粒的构建[J].陕西师范大学学报,2006,34(3):80-83. |
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| 作者姓名： | 张进安 杨章民 |
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| 作者单位： | 西安交通大学第一附属医院，陕西师范大学生命科学院，爱默蕾大学及退伍军人医疗中心内分泌代谢科，爱默蕾大学及退伍军人医疗中心内分泌代谢科，爱默蕾大学及退伍军人医疗中心内分泌代谢科 陕西西安710061，爱默蕾大学及退伍军人医疗中心内分泌代谢科，美国亚特兰大30033，陕西西安710062，美国亚特兰大30033，美国亚特兰大30033，美国亚特兰大30033 |
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| 摘 要： | 以表达人CTLA4Ig(hCTLA4Ig)的原核质粒为基础,采用限制性DNA内切酶,DNA连接酶等基因重组技术,构建了能表达该蛋白的真核质粒,ELISA法检出了受转染的真核细胞培养上清液中的蛋白表达.在此基础上构建了含hCTLA4Ig的穿梭质粒.
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| 关 键 词： | 质粒构建 真核表达 基因治疗 |
| 文章编号： | 1672-4291（2006）03-0080-04 |
| 收稿时间： | 2005-10-10 |
| 修稿时间： | 2005年10月10 |
Construction of eukaryotic expression plasmid and pShuttle plasmid containing CTLA4Ig gene |
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| Peter M.Thule,Darin E.Olson,Adam G.Campbel,ZHANG Jin-an,YANG Zhang-min,Peter M.Thule,Darin E.Olson,Adam G.Campbel.Construction of eukaryotic expression plasmid and pShuttle plasmid containing CTLA4Ig gene[J].Journal of Shaanxi Normal University: Nat Sci Ed,2006,34(3):80-83. |
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| Authors: | Peter MThule Darin EOlson Adam GCampbel ZHANG Jin-an YANG Zhang-min Peter MThule Darin EOlson Adam GCampbel |
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| Abstract: | On the basis of the availability of the prokaryotic expression plasmid which expresses human CTLA4Ig(hCTA4Ig),the eukaryotic plasmid harboring hCTLA4Ig was constructed by using recombinant DNA technique.The recombinant eukaryotic expression plasmid was identified by digestion of restricted enzymes and sequencing.The protein of hCTLA4Ig was detected by ELISA in the conditioned medium after the plasmid was transfered into 293 cells.Then hCTLA4Ig gene fragment together with eukaryotic promotor was cut from eukaryotic expression plasmid and was inserted into plasmid pShuttle.Restriction enzyme analysis showed that pShuttle/hCTLA4Ig has been successfully obtained,and it lays a foundation for CTLA4Ig gene therapy. |
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| Keywords: | construction of plasmid eukaryotic expression gene therapy |
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